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验证数据展示

  • IHC staining of human colon cancer using 68549-1-Ig

    IHC staining of human colon cancer using 68549-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68549-1-Ig (SUCLA2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of Caco-2 using 68549-1-Ig

    IF Staining of Caco-2 using 68549-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using SUCLA2 antibody (68549-1-Ig, Clone: 2C7E4 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

  • WB analysis using 68549-1-Ig

    WB analysis using 68549-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68549-1-Ig (SUCLA2 antibody) at dilution of 1:9900 incubated at room temperature for 1.5 hours.

  • WB analysis using 68549-1-Ig

    WB analysis using 68549-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68549-1-Ig (SUCLA2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 68549-1-Ig

    IF Staining of HepG2 using 68549-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SUCLA2 antibody (68549-1-Ig, Clone: 2C7E4 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red).

SUCLA2 Monoclonal antibody

SUCLA2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68549-1-Ig

产品说明书

CloneNo. 2C7E4

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

SCS betaA, EC:6.2.1.5, ATP-specific succinyl-CoA synthetase subunit beta, A-SCS, A BETA

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells, mouse brain tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inCaco-2 cells, HepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68549-1-Ig targets SUCLA2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 SUCLA2 fusion protein Ag17967 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 succinate-CoA ligase, ADP-forming, beta subunit
别名 SCS betaA, EC:6.2.1.5, ATP-specific succinyl-CoA synthetase subunit beta, A-SCS, A BETA
计算分子量 463 aa, 50 kDa
观测分子量 48-50 kDa
GenBank蛋白编号BC027587
基因名称 SUCLA2
Gene ID (NCBI) 8803
RRIDAB_3670383
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9P2R7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SUCLA2, β subunit of succinate-CoA ligase, contributes to energy production in mitochondria through the succinate-CoA ligase enzyme. Succinate-CoA ligase catalyses the reversible conversion of succinyl-CoA and ADP or GDP to succinate and ATP or GTP. SUCLA2 mutations cause global protein succinylation and mitochondrial DNA depletion (PMID: 33230181, PMID: 20301762). Mutations in SUCLA2 are also associated with SUCLA2-related mitochondrial DNA depletion syndrome, leigh syndrome, down syndrome (PMID: 23010432).

实验方案

Product Specific Protocols
WB protocol for SUCLA2 antibody 68549-1-IgDownload protocol
IHC protocol for SUCLA2 antibody 68549-1-IgDownload protocol
IF protocol for SUCLA2 antibody 68549-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols