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验证数据展示

  • WB analysis using 68551-1-Ig

    WB analysis using 68551-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68551-1-Ig (FMNL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of hTERT-RPE1 using 68551-1-Ig

    WB analysis of hTERT-RPE1 using 68551-1-Ig

    hTERT-RPE1 cells were subjected to SDS PAGE followed by western blot with 68551-1-Ig (FMNL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

FMNL2 Monoclonal antibody

FMNL2 Monoclonal Antibody for WB, ELISA
Cat No. 68551-1-Ig

产品说明书

CloneNo. 3E8C12

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

WB, ELISA

别名

FHOD2, FLJ37546, FMNL2, formin like 2, Formin like protein 2, KIAA1902

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, hTERT-RPE1 cells, HEK-293 cells, A549 cells, HepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68551-1-Ig targets FMNL2 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 FMNL2 fusion protein Ag34164 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 formin-like 2
别名 FHOD2, FLJ37546, FMNL2, formin like 2, Formin like protein 2, KIAA1902
观测分子量140-150 kDa
GenBank蛋白编号BC167159
基因名称 FMNL2
Gene ID (NCBI) 114793
RRIDAB_3085255
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96PY5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Formin-like 2 (FMNL2), also known as FRL3 or FHOD2, a member of the diaphanous-related formins, contains a GTPase-binding domain and autoregulatory domains, and is proposed to function as a downstream effector of Rho family guanosine triphosphatases (GTPases) (PMID:15950879). FMNL2 mRNA is expressed in many normal tissues and dysregulated in several cancers such as colorectal cancer, melanoma and involved in invasive behaviours and progression of cancer cells.

实验方案

Product Specific Protocols
WB protocol for FMNL2 antibody 68551-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols