验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, MOLT-4 cells |
| Positive IP detected in | Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68558-1-Ig targets HELIOS in WB, IP, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 经测试反应性 | Human |
| 免疫原 |
CatNo: Ag4357 Product name: Recombinant human IKZF2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 227-526 aa of BC028936 Sequence: NNISLVPFERPAVIEKLTGNMGKRKSSTPQKFVGEKLMRFSYPDIHFDMNLTYEKEAELMQSHMMDQAINNAITYLGAEALHPLMQHPPSTIAEVAPVISSAYSQVYHPNRIERPISRETADSHENNMDGPISLIRPKSRPQEREASPSNSCLDSTDSESSHDDHQSYQGHPALNPKRKQSPAYMKEDVKALDTTKAPKGSLKDIYKVFNGEGEQIRAFKCEHCRVLFLDHVMYTIHMGCHGYRDPLECNICGYRSQDRYEFSSHIVRGEHTFH 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | IKAROS family zinc finger 2 (Helios) |
| 别名 | HELIOS, IKZF2, Zinc finger protein Helios, ZNF1A2, ZNFN1A2 |
| 计算分子量 | 500 aa, 57 kDa |
| 观测分子量 | 65 kDa |
| GenBank蛋白编号 | BC028936 |
| 基因名称 | HELIOS |
| Gene ID (NCBI) | 22807 |
| RRID | AB_3085260 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9UKS7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IKZF2 (Helios), a member of the Ikaros transcription factor family, is expressed in a majority of Treg cells and some T cell and lymphocyte subsets. The expression of IKZF2 is associated with T cell activation and cellular division and is suggested a suitable marker to distinguish natural and induced Treg (PMID:21918685). IKZF2 plays a crucial role in maintaining function and stability of Treg cells, and IKZF2 deficiency reduces tumor growth in mice (PMID: 36863346).
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for HELIOS antibody 68558-1-Ig | Download protocol |
| WB protocol for HELIOS antibody 68558-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
