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HELIOS Monoclonal antibody

HELIOS Monoclonal Antibody for WB, IP, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1F1F10

Cat No : 68558-1-Ig

Print datasheet

Synonyms

HELIOS, IKZF2, Zinc finger protein Helios, ZNF1A2, ZNFN1A2

验证数据展示

  • WB analysis of Jurkat using 68558-1-Ig

    WB analysis of Jurkat using 68558-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 68558-1-Ig (HELIOS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 68558-1-Ig

    IP experiment of Jurkat using 68558-1-Ig

    IP result of anti-HELIOS (IP:68558-1-Ig, 4ug; Detection:68558-1-Ig 1:3000) with Jurkat cells lysate 1560 ug.

经过测试的应用

Positive WB detected inJurkat cells, MOLT-4 cells
Positive IP detected inJurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68558-1-Ig targets HELIOS in WB, IP, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IP, ELISA Application Description
Tested Reactivity Human
Immunogen HELIOS fusion protein Ag4357 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name IKAROS family zinc finger 2 (Helios)
Synonyms HELIOS, IKZF2, Zinc finger protein Helios, ZNF1A2, ZNFN1A2
Calculated Molecular Weight 500 aa, 57 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC028936
Gene Symbol HELIOS
Gene ID (NCBI) 22807
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UKS7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IKZF2 (Helios), a member of the Ikaros transcription factor family, is expressed in a majority of Treg cells and some T cell and lymphocyte subsets. The expression of IKZF2 is associated with T cell activation and cellular division and is suggested a suitable marker to distinguish natural and induced Treg (PMID:21918685). IKZF2 plays a crucial role in maintaining function and stability of Treg cells, and IKZF2 deficiency reduces tumor growth in mice (PMID: 36863346).

实验方案

Product Specific Protocols
WB protocol for HELIOS antibody 68558-1-IgDownload protocol
IP protocol for HELIOS antibody 68558-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols