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HELIOS Monoclonal antibody, PBS Only

HELIOS Monoclonal Antibody for WB, IP, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F1F10

Cat no : 68558-1-PBS

Print datasheet

Synonyms

HELIOS, IKZF2, Zinc finger protein Helios, ZNF1A2, ZNFN1A2

验证数据展示

  • WB analysis of Jurkat using 68558-1-Ig (same clone as 68558-1-PBS)

    WB analysis of Jurkat using 68558-1-Ig (same clone as 68558-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 68558-1-Ig (HELIOS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68558-1-PBS in a different storage buffer formulation.

  • IP experiment of Jurkat using 68558-1-Ig (same clone as 68558-1-PBS)

    IP experiment of Jurkat using 68558-1-Ig (same clone as 68558-1-PBS)

    IP result of anti-HELIOS (IP:68558-1-Ig, 4ug; Detection:68558-1-Ig 1:3000) with Jurkat cells lysate 1560 ug. This data was developed using the same antibody clone with 68558-1-PBS in a different storage buffer formulation.

产品信息

68558-1-PBS targets HELIOS in WB, IP, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IP, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen HELIOS fusion protein Ag4357 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name IKAROS family zinc finger 2 (Helios)
Synonyms HELIOS, IKZF2, Zinc finger protein Helios, ZNF1A2, ZNFN1A2
Calculated Molecular Weight 500 aa, 57 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC028936
Gene Symbol HELIOS
Gene ID (NCBI) 22807
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UKS7
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IKZF2 (Helios), a member of the Ikaros transcription factor family, is expressed in a majority of Treg cells and some T cell and lymphocyte subsets. The expression of IKZF2 is associated with T cell activation and cellular division and is suggested a suitable marker to distinguish natural and induced Treg (PMID:21918685). IKZF2 plays a crucial role in maintaining function and stability of Treg cells, and IKZF2 deficiency reduces tumor growth in mice (PMID: 36863346).