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验证数据展示

  • WB analysis of Jurkat using 68558-1-Ig

    WB analysis of Jurkat using 68558-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 68558-1-Ig (HELIOS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 68558-1-Ig

    IP experiment of Jurkat using 68558-1-Ig

    IP result of anti-HELIOS (IP:68558-1-Ig, 4ug; Detection:68558-1-Ig 1:3000) with Jurkat cells lysate 1560 ug.

HELIOS Monoclonal antibody

HELIOS Monoclonal Antibody for WB, IP, ELISA
Cat No. 68558-1-Ig

产品说明书

CloneNo. 1F1F10

宿主/亚型

Mouse / IgG2b

种属反应性

Human

应用

WB, IP, ELISA

别名

HELIOS, IKZF2, Zinc finger protein Helios, ZNF1A2, ZNFN1A2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, MOLT-4 cells
Positive IP detected inJurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68558-1-Ig targets HELIOS in WB, IP, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 Human
免疫原 HELIOS fusion protein Ag4357 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 IKAROS family zinc finger 2 (Helios)
别名 HELIOS, IKZF2, Zinc finger protein Helios, ZNF1A2, ZNFN1A2
计算分子量 500 aa, 57 kDa
观测分子量 65 kDa
GenBank蛋白编号BC028936
基因名称 HELIOS
Gene ID (NCBI) 22807
RRIDAB_3085260
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9UKS7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IKZF2 (Helios), a member of the Ikaros transcription factor family, is expressed in a majority of Treg cells and some T cell and lymphocyte subsets. The expression of IKZF2 is associated with T cell activation and cellular division and is suggested a suitable marker to distinguish natural and induced Treg (PMID:21918685). IKZF2 plays a crucial role in maintaining function and stability of Treg cells, and IKZF2 deficiency reduces tumor growth in mice (PMID: 36863346).

实验方案

Product Specific Protocols
WB protocol for HELIOS antibody 68558-1-IgDownload protocol
IP protocol for HELIOS antibody 68558-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols