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验证数据展示

  • WB analysis using 68560-1-Ig

    WB analysis using 68560-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68560-1-Ig (RAD51AP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

RAD51AP1 Monoclonal antibody

RAD51AP1 Monoclonal Antibody for WB, ELISA
Cat No. 68560-1-Ig

产品说明书

CloneNo. 2D8B1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, ELISA

别名

RAD51-interacting protein, RAD51-associated protein 1, RAD51 interacting protein, RAD51 associated protein 1, PIR51

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, SW480 cells, NCCIT cells, Daudi cells, HaCaT cells, Jurkat cells, K-562 cells, NIH/3T3 cells, 4T1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68560-1-Ig targets RAD51AP1 in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原 RAD51AP1 fusion protein Ag28385 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 RAD51 associated protein 1
别名 RAD51-interacting protein, RAD51-associated protein 1, RAD51 interacting protein, RAD51 associated protein 1, PIR51
计算分子量 38 kDa
观测分子量38-40 kDa
GenBank蛋白编号BC016330
基因名称 RAD51AP1
Gene ID (NCBI) 10635
RRIDAB_3085262
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96B01
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Homologous recombination (HR) is an important pathway for the elimination of DNA double-strand breaks (DSBs) and other deleterious lesions from chromosomes. The eukaryotic HR reaction is mediated by RAD51 [PMID:12456786]. RAD51AP1 (RAD51 associated protein 1), so named because it can interact with human RAD51, is a vertebrate-specific protein that enhances the ability of RAD51 to mediate the D-loop reaction. RAD51AP1 knockdown by siRNA leads to cellular sensitivity to DNA damaging agents and elevated DNA damage-induced chromatid breaks. Enhancement of the RAD51 recombinase activity by RAD51AP1 is contingent upon the physical interaction of the two HR factors [PMID:17996710,17996711].

实验方案

Product Specific Protocols
WB protocol for RAD51AP1 antibody 68560-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols