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RAD51AP1 Monoclonal antibody

RAD51AP1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2D8B1

Cat no : 68560-1-Ig

Print datasheet

Synonyms

PIR51, RAD51 associated protein 1, RAD51 interacting protein, RAD51AP1

验证数据展示

  • WB analysis using 68560-1-Ig

    WB analysis using 68560-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68560-1-Ig (RAD51AP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, SW480 cells, NCCIT cells, Daudi cells, HaCaT cells, Jurkat cells, K-562 cells, NIH/3T3 cells, 4T1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68560-1-Ig targets RAD51AP1 in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen RAD51AP1 fusion protein Ag28385 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name RAD51 associated protein 1
Synonyms PIR51, RAD51 associated protein 1, RAD51 interacting protein, RAD51AP1
Calculated Molecular Weight 38 kDa
Observed Molecular Weight38-40 kDa
GenBank Accession NumberBC016330
Gene Symbol RAD51AP1
Gene ID (NCBI) 10635
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96B01
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Homologous recombination (HR) is an important pathway for the elimination of DNA double-strand breaks (DSBs) and other deleterious lesions from chromosomes. The eukaryotic HR reaction is mediated by RAD51 [PMID:12456786]. RAD51AP1 (RAD51 associated protein 1), so named because it can interact with human RAD51, is a vertebrate-specific protein that enhances the ability of RAD51 to mediate the D-loop reaction. RAD51AP1 knockdown by siRNA leads to cellular sensitivity to DNA damaging agents and elevated DNA damage-induced chromatid breaks. Enhancement of the RAD51 recombinase activity by RAD51AP1 is contingent upon the physical interaction of the two HR factors [PMID:17996710,17996711].

实验方案

Product Specific Protocols
WB protocol for RAD51AP1 antibody 68560-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols