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RAD51AP1 Monoclonal antibody, PBS Only

RAD51AP1 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D8B1

Cat no : 68560-1-PBS

Print datasheet

Synonyms

PIR51, RAD51 associated protein 1, RAD51 interacting protein, RAD51AP1

验证数据展示

  • WB analysis using 68560-1-Ig (same clone as 68560-1-PBS)

    WB analysis using 68560-1-Ig (same clone as 68560-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68560-1-Ig (RAD51AP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68560-1-PBS in a different storage buffer formulation.

产品信息

68560-1-PBS targets RAD51AP1 in WB, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity Human, mouse
Immunogen RAD51AP1 fusion protein Ag28385 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name RAD51 associated protein 1
Synonyms PIR51, RAD51 associated protein 1, RAD51 interacting protein, RAD51AP1
Calculated Molecular Weight 38 kDa
Observed Molecular Weight38-40 kDa
GenBank Accession NumberBC016330
Gene Symbol RAD51AP1
Gene ID (NCBI) 10635
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96B01
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Homologous recombination (HR) is an important pathway for the elimination of DNA double-strand breaks (DSBs) and other deleterious lesions from chromosomes. The eukaryotic HR reaction is mediated by RAD51 [PMID:12456786]. RAD51AP1 (RAD51 associated protein 1), so named because it can interact with human RAD51, is a vertebrate-specific protein that enhances the ability of RAD51 to mediate the D-loop reaction. RAD51AP1 knockdown by siRNA leads to cellular sensitivity to DNA damaging agents and elevated DNA damage-induced chromatid breaks. Enhancement of the RAD51 recombinase activity by RAD51AP1 is contingent upon the physical interaction of the two HR factors [PMID:17996710,17996711].