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验证数据展示

  • IF Staining of HeLa using 68582-1-Ig

    IF Staining of HeLa using 68582-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PTPIP51 antibody (68582-1-Ig, Clone: 1G11E2 ) at dilution of 1:476 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

  • WB analysis using 68582-1-Ig

    WB analysis using 68582-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68582-1-Ig (PTPIP51 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

PTPIP51 Monoclonal antibody

PTPIP51 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68582-1-Ig

产品说明书

CloneNo. 1G11E2

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

FAM82A2, 1G11E2, hucep-10, hRMD-3, hRMD 3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, HepG2 cells, HuH-7 cells, HT-29 cells, HEK-293 cells, K-562 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:238-1:952
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68582-1-Ig targets PTPIP51 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 PTPIP51 fusion protein Ag15135 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 family with sequence similarity 82, member A2
别名 FAM82A2, 1G11E2, hucep-10, hRMD-3, hRMD 3
计算分子量 470 aa, 52 kDa
观测分子量52-60 kDa
GenBank蛋白编号BC008970
基因名称 PTPIP51
Gene ID (NCBI) 55177
RRIDAB_3085282
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96TC7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein tyrosine phosphatase-interacting protein 51 (PTPIP51),also known as RMDN3, is a mitochondrial membrane-anchored protein that interacts with ER membrane-bound VAPB. The VAPB-PTPIP51 interaction impacts on intracellular Ca2+ handling (PMID: 22131369, 28345618). PTPIP51 participates in multiple cellular processes, and dysfunction of PTPIP51 is implicated in diseases such as cancer and neurodegenerative disorders (PMID: 28345618).The known initiation sites in the Ptpip51 gene would lead to molecular protein masses of 52, 38 and 30 kDa (PMID: 18854601,19844996).

实验方案

Product Specific Protocols
WB protocol for PTPIP51 antibody 68582-1-IgDownload protocol
IF protocol for PTPIP51 antibody 68582-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols