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验证数据展示

  • WB analysis using 68586-1-Ig

    WB analysis using 68586-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68586-1-Ig (BCAT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • IF Staining of A431 using 68586-1-Ig

    IF Staining of A431 using 68586-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using BCAT2 antibody (68586-1-Ig, Clone: 1A5A5 ) at dilution of 1:1130 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

BCAT2 Monoclonal antibody

BCAT2 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68586-1-Ig

产品说明书

CloneNo. 1A5A5

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

PP18, Placental protein 18, ECA40, EC:2.6.1.42, Branched-chain-amino-acid aminotransferase, mitochondrial

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HCT 116 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, Raji cells, mouse testis tissue
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:565-1:2260
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68586-1-Ig targets BCAT2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 BCAT2 fusion protein Ag9595 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 branched chain aminotransferase 2, mitochondrial
别名 PP18, Placental protein 18, ECA40, EC:2.6.1.42, Branched-chain-amino-acid aminotransferase, mitochondrial
计算分子量 44 kDa
观测分子量38 kDa
GenBank蛋白编号BC001900
基因名称 BCAT2
Gene ID (NCBI) 587
RRIDAB_3085285
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO15382
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Branched-chain amino acid transaminase 2 (BCAT2) is an important enzyme in branched-chain amino acids (BCAAs) catabolism that reversibly catalyzes the initial step of BCAA degradation to branched-chain acyl-CoA. In humans, BCAT2 encodes a mitochondrial protein that is ubiquitously expressed in all organs (except hepatocytes) (PMID:32467562). Moreover, other reports showed that overexpressed BCAT2 in pancreatic ductal adenocarcinoma (PDAC) cells enhanced cell proliferation and survival in vitro and in vivo (PMID:35663242).

实验方案

Product Specific Protocols
WB protocol for BCAT2 antibody 68586-1-IgDownload protocol
IF protocol for BCAT2 antibody 68586-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols