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BCAT2 Monoclonal antibody, PBS Only (Capture)

BCAT2 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A5A5

Cat no : 68586-1-PBS

Print datasheet

Synonyms

PP18, Placental protein 18, ECA40, EC:2.6.1.42, Branched-chain-amino-acid aminotransferase, mitochondrial

验证数据展示

  • WB analysis using 68586-1-Ig (same clone as 68586-1-PBS)

    WB analysis using 68586-1-Ig (same clone as 68586-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68586-1-Ig (BCAT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68586-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 68586-1-Ig (same clone as 68586-1-PBS)

    IF Staining of A431 using 68586-1-Ig (same clone as 68586-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using BCAT2 antibody (68586-1-Ig, Clone: 1A5A5 ) at dilution of 1:1130 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 68586-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50268-2

    Cytometric bead array standard curve of MP50268-2

    Cytometric bead array standard curve of MP50268-2, BCAT2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68586-1-PBS. Detection antibody: 68586-4-PBS. Standard:Ag9595. Range: 0.781-100 ng/mL.

产品信息

68586-1-PBS targets BCAT2 as part of a matched antibody pair:

MP50268-2: 68586-1-PBS capture and 68586-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen BCAT2 fusion protein Ag9595 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name branched chain aminotransferase 2, mitochondrial
Synonyms PP18, Placental protein 18, ECA40, EC:2.6.1.42, Branched-chain-amino-acid aminotransferase, mitochondrial
Calculated Molecular Weight 44 kDa
Observed Molecular Weight38 kDa
GenBank Accession NumberBC001900
Gene Symbol BCAT2
Gene ID (NCBI) 587
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO15382
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Branched-chain amino acid transaminase 2 (BCAT2) is an important enzyme in branched-chain amino acids (BCAAs) catabolism that reversibly catalyzes the initial step of BCAA degradation to branched-chain acyl-CoA. In humans, BCAT2 encodes a mitochondrial protein that is ubiquitously expressed in all organs (except hepatocytes) (PMID:32467562). Moreover, other reports showed that overexpressed BCAT2 in pancreatic ductal adenocarcinoma (PDAC) cells enhanced cell proliferation and survival in vitro and in vivo (PMID:35663242).