BCAT2 Monoclonal antibody
BCAT2 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, mouse
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
1A5A5
验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, HCT 116 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, Raji cells, mouse testis tissue |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
68586-1-Ig targets BCAT2 in WB, ELISA applications and shows reactivity with Human, mouse samples.
Tested Applications | WB, ELISA Application Description |
Tested Reactivity | Human, mouse |
Immunogen | BCAT2 fusion protein Ag9595 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | branched chain aminotransferase 2, mitochondrial |
Synonyms | BCAT2, ECA40, Placental protein 18, PP18 |
Calculated Molecular Weight | 44 kDa |
Observed Molecular Weight | 38 kDa |
GenBank Accession Number | BC001900 |
Gene Symbol | BCAT2 |
Gene ID (NCBI) | 587 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | O15382 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Branched-chain amino acid transaminase 2 (BCAT2) is an important enzyme in branched-chain amino acids (BCAAs) catabolism that reversibly catalyzes the initial step of BCAA degradation to branched-chain acyl-CoA. In humans, BCAT2 encodes a mitochondrial protein that is ubiquitously expressed in all organs (except hepatocytes) (PMID:32467562). Moreover, other reports showed that overexpressed BCAT2 in pancreatic ductal adenocarcinoma (PDAC) cells enhanced cell proliferation and survival in vitro and in vivo (PMID:35663242).
实验方案
Product Specific Protocols | |
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WB protocol for BCAT2 antibody 68586-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |