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验证数据展示

  • WB analysis using 68626-1-Ig

    WB analysis using 68626-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68626-1-Ig (PPM1F antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis using 68626-1-Ig

    WB analysis using 68626-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68626-1-Ig (PPM1F antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

PPM1F Monoclonal antibody

PPM1F Monoclonal Antibody for WB, ELISA
Cat No. 68626-1-Ig

产品说明书

CloneNo. 2A2E8

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

WB, ELISA

别名

CaM kinase phosphatase, CaMKPase, FEM 2, hFEM 2, KIAA0015, Partner of PIX 2, POPX2, PPM1F, Protein fem 2 homolog, Protein phosphatase 1F

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inU2OS cells, A375 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, human peripheral blood platelets

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68626-1-Ig targets PPM1F in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 PPM1F fusion protein Ag10316 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 protein phosphatase 1F (PP2C domain containing)
别名 CaM kinase phosphatase, CaMKPase, FEM 2, hFEM 2, KIAA0015, Partner of PIX 2, POPX2, PPM1F, Protein fem 2 homolog, Protein phosphatase 1F
计算分子量 453 aa, 50 kDa
观测分子量50 kDa
GenBank蛋白编号BC071989
基因名称 PPM1F
Gene ID (NCBI) 9647
RRIDAB_3085318
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP49593
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mg2+/Mn2+-dependent protein phosphatase 1F (PPM1F) belongs to the PP2C family of Ser/Thr protein phosphatases and is the critical enzyme responsible for dephosphorylating the threonine motif. PPM1F is ubiquitous in various tissues and organs (PMID: 26498692). High expression of PPM1F was positively associated with poor survival and tumor recurrence in patients with cancers and PPM1F might be a potential biomarker in HCC patients (PMID: 30470261).

实验方案

Product Specific Protocols
WB protocol for PPM1F antibody 68626-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols