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PPM1F Monoclonal antibody, PBS Only

PPM1F Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A2E8

Cat no : 68626-1-PBS

Print datasheet

Synonyms

CaM kinase phosphatase, CaMKPase, FEM 2, hFEM 2, KIAA0015, Partner of PIX 2, POPX2, PPM1F, Protein fem 2 homolog, Protein phosphatase 1F

验证数据展示

  • WB analysis using 68626-1-Ig (same clone as 68626-1-PBS)

    WB analysis using 68626-1-Ig (same clone as 68626-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68626-1-Ig (PPM1F antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68626-1-PBS in a different storage buffer formulation.

  • WB analysis using 68626-1-Ig (same clone as 68626-1-PBS)

    WB analysis using 68626-1-Ig (same clone as 68626-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68626-1-Ig (PPM1F antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68626-1-PBS in a different storage buffer formulation.

产品信息

68626-1-PBS targets PPM1F in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen PPM1F fusion protein Ag10316 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name protein phosphatase 1F (PP2C domain containing)
Synonyms CaM kinase phosphatase, CaMKPase, FEM 2, hFEM 2, KIAA0015, Partner of PIX 2, POPX2, PPM1F, Protein fem 2 homolog, Protein phosphatase 1F
Calculated Molecular Weight 453 aa, 50 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC071989
Gene Symbol PPM1F
Gene ID (NCBI) 9647
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49593
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mg2+/Mn2+-dependent protein phosphatase 1F (PPM1F) belongs to the PP2C family of Ser/Thr protein phosphatases and is the critical enzyme responsible for dephosphorylating the threonine motif. PPM1F is ubiquitous in various tissues and organs (PMID: 26498692). High expression of PPM1F was positively associated with poor survival and tumor recurrence in patients with cancers and PPM1F might be a potential biomarker in HCC patients (PMID: 30470261).