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PPM1F Monoclonal antibody

PPM1F Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2A2E8

Cat no : 68626-1-Ig

Print datasheet

Synonyms

CaM kinase phosphatase, CaMKPase, FEM 2, hFEM 2, KIAA0015, Partner of PIX 2, POPX2, PPM1F, Protein fem 2 homolog, Protein phosphatase 1F

验证数据展示

  • WB analysis using 68626-1-Ig

    WB analysis using 68626-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68626-1-Ig (PPM1F antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis using 68626-1-Ig

    WB analysis using 68626-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68626-1-Ig (PPM1F antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inU2OS cells, A375 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, human peripheral blood platelets

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68626-1-Ig targets PPM1F in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen PPM1F fusion protein Ag10316 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name protein phosphatase 1F (PP2C domain containing)
Synonyms CaM kinase phosphatase, CaMKPase, FEM 2, hFEM 2, KIAA0015, Partner of PIX 2, POPX2, PPM1F, Protein fem 2 homolog, Protein phosphatase 1F
Calculated Molecular Weight 453 aa, 50 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC071989
Gene Symbol PPM1F
Gene ID (NCBI) 9647
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49593
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mg2+/Mn2+-dependent protein phosphatase 1F (PPM1F) belongs to the PP2C family of Ser/Thr protein phosphatases and is the critical enzyme responsible for dephosphorylating the threonine motif. PPM1F is ubiquitous in various tissues and organs (PMID: 26498692). High expression of PPM1F was positively associated with poor survival and tumor recurrence in patients with cancers and PPM1F might be a potential biomarker in HCC patients (PMID: 30470261).

实验方案

Product Specific Protocols
WB protocol for PPM1F antibody 68626-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols