验证数据展示
经过测试的应用
| Positive Dot Blot detected in | RNA |
推荐稀释比
| Application | Dilution |
|---|---|
| DOT BLOT | DOT BLOT : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68636-1-Ig targets 1-Methyladenosine in Dot Blot, ELISA applications and shows reactivity with chemical compound samples.
| Tested Applications | Dot Blot, ELISA Application Description |
| Tested Reactivity | chemical compound |
| Immunogen | PTG 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | 1-Methyladenosine |
| Synonyms | N1-methyladenosine, m1A |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_3085325 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
1-Methyladenosine, also known as m1A, belongs to the class of organic compounds known as purine nucleosides. M1A is important for the tRNA 3-dimensional structure, and it is also important for structure stabilization of tRNA due to its positive electrostatic charge and assists in correct folding of tRNA. Recently evidence suggests that m1A in tRNA is under reversible regulation that is installed by a tRNA m1A methyltransferase (MTase) and is erased by AlkB Homolog 1 (ALKBH1) and AlkB Homolog 3 (ALKBH3).
Protocol for Dot Blot:
https://www.ptglab.com/protocol/68636-1-IgDotBlot.pdf