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验证数据展示

  • Dot Blot experiment of RNA using 68636-1-Ig (same clone as 68636-1-PBS)

    Dot Blot experiment of RNA using 68636-1-Ig (same clone as 68636-1-PBS)

    Total RNA was isolated from HeLa cell line and was dotted to NC membrane at different amount as indicated above the dots. The membrane was blocked with 1% BSA and blotted with m1A (1-Methyladenosine) antibody 68636-1-Ig at 1:2000 followed by incubation of HRP-goat anti-mouse secondary antibody. Signal was developed by ECL substrate. A parallel dot blot was performed using Mouse IgG2a isotype control Monoclonal antibody 66360-2-Ig at the same dose. This data was developed using the same antibody clone with 68636-1-PBS in a different storage buffer formulation.

Na 1-Methyladenosine Monoclonal antibody, PBS Only

1-Methyladenosine Monoclonal Antibody for Dot Blot, Indirect ELISA
Cat No. 68636-1-PBS

产品说明书

CloneNo. 2F2H8

宿主/亚型

Mouse / IgG2a

种属反应性

chemical compound

应用

Dot Blot, Indirect ELISA

别名

N1-methyladenosine, m1A

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68636-1-PBS targets 1-Methyladenosine in Dot Blot, Indirect ELISA applications and shows reactivity with chemical compound samples.

经测试应用 Dot Blot, Indirect ELISA Application Description
经测试反应性 chemical compound
免疫原 PTG 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 1-Methyladenosine
别名 N1-methyladenosine, m1A
基因名称
Gene ID (NCBI)
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

1-Methyladenosine, also known as m1A, belongs to the class of organic compounds known as purine nucleosides. M1A is important for the tRNA 3-dimensional structure, and it is also important for structure stabilization of tRNA due to its positive electrostatic charge and assists in correct folding of tRNA. Recently evidence suggests that m1A in tRNA is under reversible regulation that is installed by a tRNA m1A methyltransferase (MTase) and is erased by AlkB Homolog 1 (ALKBH1) and AlkB Homolog 3 (ALKBH3).

Protocol for Dot Blot:
https://www.ptglab.com/protocol/68636-1-IgDotBlot.pdf