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Na 1-Methyladenosine Monoclonal antibody, PBS Only

1-Methyladenosine Monoclonal Antibody for Dot Blot, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

chemical compound

Applications

Dot Blot, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F2H8

Cat no : 68636-1-PBS

Print datasheet

Synonyms

N1-methyladenosine, m1A

验证数据展示

  • Dot Blot experiment of RNA using 68636-1-Ig (same clone as 68636-1-PBS)

    Dot Blot experiment of RNA using 68636-1-Ig (same clone as 68636-1-PBS)

    Total RNA was isolated from HeLa cell line and was dotted to NC membrane at different amount as indicated above the dots. The membrane was blocked with 1% BSA and blotted with m1A (1-Methyladenosine) antibody 68636-1-Ig at 1:2000 followed by incubation of HRP-goat anti-mouse secondary antibody. Signal was developed by ECL substrate. A parallel dot blot was performed using Mouse IgG2a isotype control Monoclonal antibody 66360-2-Ig at the same dose. This data was developed using the same antibody clone with 68636-1-PBS in a different storage buffer formulation.

产品信息

68636-1-PBS targets 1-Methyladenosine in Dot Blot, Indirect ELISA applications and shows reactivity with chemical compound samples.

Tested Applications Dot Blot, Indirect ELISA Application Description
Tested Reactivity chemical compound
Immunogen PTG 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name 1-Methyladenosine
Synonyms N1-methyladenosine, m1A
Gene Symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

1-Methyladenosine, also known as m1A, belongs to the class of organic compounds known as purine nucleosides. M1A is important for the tRNA 3-dimensional structure, and it is also important for structure stabilization of tRNA due to its positive electrostatic charge and assists in correct folding of tRNA. Recently evidence suggests that m1A in tRNA is under reversible regulation that is installed by a tRNA m1A methyltransferase (MTase) and is erased by AlkB Homolog 1 (ALKBH1) and AlkB Homolog 3 (ALKBH3).

Protocol for Dot Blot:
https://www.ptglab.com/protocol/68636-1-IgDotBlot.pdf