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1-Methyladenosine Monoclonal antibody

1-Methyladenosine Monoclonal Antibody for Dot Blot, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

chemical compound

Applications

Dot Blot, ELISA

Conjugate

Unconjugated

CloneNo.

2F2H8

Cat no : 68636-1-Ig

Print datasheet

Synonyms

N1-methyladenosine, m1A

验证数据展示

  • Dot Blot experiment of RNA using 68636-1-Ig

    Dot Blot experiment of RNA using 68636-1-Ig

    Total RNA was isolated from HeLa cell line and was dotted to NC membrane at different amount as indicated above the dots. The membrane was blocked with 1% BSA and blotted with m1A (1-Methyladenosine) antibody 68636-1-Ig at 1:2000 followed by incubation of HRP-goat anti-mouse secondary antibody. Signal was developed by ECL substrate. A parallel dot blot was performed using Mouse IgG2a isotype control Monoclonal antibody 66360-2-Ig at the same dose.

经过测试的应用

Positive Dot Blot detected inRNA

推荐稀释比

ApplicationDilution
DOT BLOTDOT BLOT : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68636-1-Ig targets 1-Methyladenosine in Dot Blot, ELISA applications and shows reactivity with chemical compound samples.

Tested Applications Dot Blot, ELISA Application Description
Tested Reactivity chemical compound
Immunogen PTG 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name 1-Methyladenosine
Synonyms N1-methyladenosine, m1A
Gene Symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

1-Methyladenosine, also known as m1A, belongs to the class of organic compounds known as purine nucleosides. M1A is important for the tRNA 3-dimensional structure, and it is also important for structure stabilization of tRNA due to its positive electrostatic charge and assists in correct folding of tRNA. Recently evidence suggests that m1A in tRNA is under reversible regulation that is installed by a tRNA m1A methyltransferase (MTase) and is erased by AlkB Homolog 1 (ALKBH1) and AlkB Homolog 3 (ALKBH3).

Protocol for Dot Blot:
https://www.ptglab.com/protocol/68636-1-IgDotBlot.pdf