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验证数据展示

  • WB analysis using 68642-1-Ig

    WB analysis using 68642-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68642-1-Ig (COG1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 68642-1-Ig

    IF Staining of U2OS using 68642-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using COG1 antibody (68642-1-Ig, Clone: 2D8D11 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • IF Staining of U2OS using 68642-1-Ig

    IF Staining of U2OS using 68642-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using COG1 antibody (68642-1-Ig, Clone: 2D8D11 ) at dilution of 1:1500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • WB analysis of LNCaP using 68642-1-Ig

    WB analysis of LNCaP using 68642-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68642-1-Ig (COG1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

COG1 Monoclonal antibody

COG1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68642-1-Ig

产品说明书

CloneNo. 2D8D11

宿主/亚型

Mouse / IgG1

种属反应性

human, rabbit

应用

WB, IF/ICC, ELISA

别名

LDLB, KIAA1381, COG complex subunit 1, CDG2G, 2D8D11

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, LNCaP cells, Jurkat cells
Positive IF/ICC detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy COG1 Ready-To-Use IHC Kit. COG1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68642-1-Ig targets COG1 in WB, IF/ICC, ELISA applications and shows reactivity with human, rabbit samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, rabbit
免疫原 COG1 fusion protein Ag33924 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 component of oligomeric golgi complex 1
别名 LDLB, KIAA1381, COG complex subunit 1, CDG2G, 2D8D11
计算分子量 109 kDa
观测分子量100-109 kDa
GenBank蛋白编号BC021985
基因名称 COG1
Gene ID (NCBI) 9382
RRIDAB_3670386
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8WTW3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COG1 is one protein of the COG complex which is a heterooctameric complex that regulates intraGolgi trafficking and the integrity of the Golgi compartment in eukaryotic cells. It is reported that this protein is required for steps in the normal medial and trans-Golgi-associated processing of glycoconjugates and plays a role in the organization of the Golgi-localized complex. COG1 has a calculated molecular weight of 109 kDa and western blot analysis detected COG1 at 100-109 kDa (PMID: 16537452).

实验方案

Product Specific Protocols
WB protocol for COG1 antibody 68642-1-IgDownload protocol
IF protocol for COG1 antibody 68642-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols