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验证数据展示

  • WB analysis using 68642-1-Ig (same clone as 68642-1-PBS)

    WB analysis using 68642-1-Ig (same clone as 68642-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68642-1-Ig (COG1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68642-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 68642-1-Ig (same clone as 68642-1-PBS)

    IF Staining of U2OS using 68642-1-Ig (same clone as 68642-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using COG1 antibody (68642-1-Ig, Clone: 2D8D11 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68642-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 68642-1-Ig (same clone as 68642-1-PBS)

    IF Staining of U2OS using 68642-1-Ig (same clone as 68642-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using COG1 antibody (68642-1-Ig, Clone: 2D8D11 ) at dilution of 1:1500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68642-1-PBS in a different storage buffer formulation.

  • WB analysis of LNCaP using 68642-1-Ig (same clone as 68642-1-PBS)

    WB analysis of LNCaP using 68642-1-Ig (same clone as 68642-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68642-1-Ig (COG1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68642-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51262-1

    Cytometric bead array standard curve of MP51262-1

    Cytometric bead array standard curve of MP51262-1, COG1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68642-1-PBS. Detection antibody: 68642-2-PBS. Standard:Ag33924. Range: 3.125-200 ng/mL.

COG1 Monoclonal antibody, PBS Only (Capture)

COG1 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 68642-1-PBS

产品说明书

CloneNo. 2D8D11

宿主/亚型

Mouse / IgG1

种属反应性

human, rabbit

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

LDLB, KIAA1381, COG complex subunit 1, CDG2G, 2D8D11

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy COG1 Ready-To-Use IHC Kit. COG1 primary antibody included.

产品信息

68642-1-PBS targets COG1 as part of a matched antibody pair:

MP51262-1: 68642-1-PBS capture and 68642-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, rabbit
免疫原 COG1 fusion protein Ag33924 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 component of oligomeric golgi complex 1
别名 LDLB, KIAA1381, COG complex subunit 1, CDG2G, 2D8D11
计算分子量 109 kDa
观测分子量100-109 kDa
GenBank蛋白编号BC021985
基因名称 COG1
Gene ID (NCBI) 9382
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8WTW3
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

COG1 is one protein of the COG complex which is a heterooctameric complex that regulates intraGolgi trafficking and the integrity of the Golgi compartment in eukaryotic cells. It is reported that this protein is required for steps in the normal medial and trans-Golgi-associated processing of glycoconjugates and plays a role in the organization of the Golgi-localized complex. COG1 has a calculated molecular weight of 109 kDa and western blot analysis detected COG1 at 100-109 kDa (PMID: 16537452).