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COG1 Monoclonal antibody

COG1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2D8D11

Cat no : 68642-1-Ig

Print datasheet

Synonyms

CDG2G, COG complex subunit 1, COG1, DKFZp762L1710, KIAA1381, LDLB

验证数据展示

  • WB analysis using 68642-1-Ig

    WB analysis using 68642-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68642-1-Ig (COG1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of LNCaP using 68642-1-Ig

    WB analysis of LNCaP using 68642-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68642-1-Ig (COG1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHepG2 cells, LNCaP cells, Jurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68642-1-Ig targets COG1 in WB, ELISA applications and shows reactivity with Human, rabbit samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, rabbit
Immunogen COG1 fusion protein Ag33924 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name component of oligomeric golgi complex 1
Synonyms CDG2G, COG complex subunit 1, COG1, DKFZp762L1710, KIAA1381, LDLB
Calculated Molecular Weight 109 kDa
Observed Molecular Weight100-109 kDa
GenBank Accession NumberBC021985
Gene Symbol COG1
Gene ID (NCBI) 9382
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8WTW3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COG1 is one protein of the COG complex which is a heterooctameric complex that regulates intraGolgi trafficking and the integrity of the Golgi compartment in eukaryotic cells. It is reported that this protein is required for steps in the normal medial and trans-Golgi-associated processing of glycoconjugates and plays a role in the organization of the Golgi-localized complex. COG1 has a calculated molecular weight of 109 kDa and western blot analysis detected COG1 at 100-109 kDa (PMID: 16537452).

实验方案

Product Specific Protocols
WB protocol for COG1 antibody 68642-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols