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验证数据展示

  • WB analysis using 68712-1-Ig

    WB analysis using 68712-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68712-1-Ig (TPX2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68712-1-Ig

    WB analysis using 68712-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68712-1-Ig (TPX2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

TPX2 Monoclonal antibody

TPX2 Monoclonal Antibody for WB, ELISA
Cat No. 68712-1-Ig

产品说明书

CloneNo. 2E10C1

宿主/亚型

Mouse / IgG2b

种属反应性

Human, mouse, rat

应用

WB, ELISA

别名

C20orf1, C20orf2, DIL 2, DIL2, FLS353, GD:C20orf1, HCA519, HCTP4, p100, Protein fls353, REPP86, Targeting protein for Xklp2, TPX2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, Jurkat cells, K-562 cells, MCF-7 cells, HeLa cells, U2OS cells, HSC-T6 cells, NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy TPX2 Ready-To-Use IHC Kit. TPX2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68712-1-Ig targets TPX2 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 TPX2 fusion protein Ag33683 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 TPX2, microtubule-associated, homolog (Xenopus laevis)
别名 C20orf1, C20orf2, DIL 2, DIL2, FLS353, GD:C20orf1, HCA519, HCTP4, p100, Protein fls353, REPP86, Targeting protein for Xklp2, TPX2
计算分子量 747 aa, 86 kDa
观测分子量 98 kDa
GenBank蛋白编号BC020207
基因名称 TPX2
Gene ID (NCBI) 22974
RRIDAB_3670405
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9ULW0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TPX2, also known as DIL2, p10, is a microtubule organization- and cell cycle-associated protein. In the early stage of mitosis, TPX2 is released in a RanGTP-dependent manner and plays a significant role in mitotic spindle formation and subsequent proper segregation of chromosomes during cell division. Furthermore, during interphase, TPX2 is involved in DNA damage response by regulation of γ-H2AX signals(PMID: 25914189). Aberrant expression of TPX2 leads to improper spindle assembly and chromosomal instability. Overexpressed in cancers, TPX2 is being established as the marker for the diagnosis and prognosis of malignancies(PMID: 24556998).

实验方案

Product Specific Protocols
WB protocol for TPX2 antibody 68712-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols