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验证数据展示

  • WB analysis using 68717-1-Ig

    WB analysis using 68717-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68717-1-Ig (KIAA0319L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

KIAA0319L Monoclonal antibody

KIAA0319L Monoclonal Antibody for WB, ELISA
Cat No. 68717-1-Ig

产品说明书

CloneNo. 2E11D9

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

WB, ELISA

别名

KIAA0319 like, KIAA0319L, KIAA1837, PKD1 like, PP791, RP4 765A10.3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, K-562 cells, U2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68717-1-Ig targets KIAA0319L in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 KIAA0319L fusion protein Ag15263 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 KIAA0319-like
别名 KIAA0319 like, KIAA0319L, KIAA1837, PKD1 like, PP791, RP4 765A10.3
计算分子量 1049 aa, 116 kDa
观测分子量140-150 kDa
GenBank蛋白编号BC031672
基因名称 KIAA0319L
Gene ID (NCBI) 79932
RRIDAB_3670410
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8IZA0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KIAA0319L (also known as AAVR) is a type-I transmembrane protein that acts as a receptor for adeno-associated virus (AAV) and is involved in adeno-associated virus infection through endocytosis system (PMID: 26814968). It has been shown that KIAA0319L is highly-glycosylated and can localise to the plasma membrane, endosomes, Golgi and trans-Golgi network (TGN) (PMID: 29991750). KIAA0319L gene has been associated with susceptibility to developmental dyslexia or reading disabilities (PMID: 19085271). The predicted molecular mass of KIAA0319L is 116 kDa, but western blot detected protein at 150 kDa in lysate from HeLa cells, likely due to glycosidic linkages (PMID: 28679762; 30962536).

实验方案

Product Specific Protocols
WB protocol for KIAA0319L antibody 68717-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols