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ADAM17 Monoclonal antibody, PBS Only (Detector)

ADAM17 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F4C2

Cat no : 68725-1-PBS

Print datasheet

Synonyms

Snake venom-like protease, EC:3.4.24.86, Disintegrin and metalloproteinase domain-containing protein 17, cSVP, ADAM 17

验证数据展示

  • WB analysis using 68725-1-Ig (same clone as 68725-1-PBS)

    WB analysis using 68725-1-Ig (same clone as 68725-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68725-1-Ig (ADAM17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68725-1-PBS in a different storage buffer formulation.

  • WB analysis using 68725-1-Ig (same clone as 68725-1-PBS)

    WB analysis using 68725-1-Ig (same clone as 68725-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68725-1-Ig (ADAM17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68725-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50392-2

    Cytometric bead array standard curve of MP50392-2

    Cytometric bead array standard curve of MP50392-2, ADAM17 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68725-2-PBS. Detection antibody: 68725-1-PBS. Standard:Ag34022. Range: 0.098-100 ng/mL.

产品信息

68725-1-PBS targets ADAM17 as part of a matched antibody pair:

MP50392-2: 68725-2-PBS capture and 68725-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen ADAM17 fusion protein Ag34022 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ADAM metallopeptidase domain 17
Synonyms Snake venom-like protease, EC:3.4.24.86, Disintegrin and metalloproteinase domain-containing protein 17, cSVP, ADAM 17
Calculated Molecular Weight 824 aa, 93 kDa
Observed Molecular Weight 90-110 kDa
GenBank Accession NumberBC136783
Gene Symbol ADAM17
Gene ID (NCBI) 6868
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP78536
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The ADAMs (A Disintegrin And Metalloprotease) are multidomain transmembrane proteins. One of the first ADAMs implicated in membrane shedding is ADAM-17, which is shown to release the active form of tumor necrosis factor (TNF)-a from its precursor (PMID:18238782). ADAM17 is also named as CSVP, TACE. The full length protein has 9 glycosylation sites, a signal peptide, propeptide and 2 isoforms produced by alternative splicing.The 120-kDa form of ADAM-17 is expressed more frequently and at higher levels in primary breast carcinomas compared with normal breast tissue(PMID:17438092).