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验证数据展示

  • WB analysis of HEK-293 using 80077-2-RR

    WB analysis of HEK-293 using 80077-2-RR

    Phosphatase inhibitor treated or λ phosphatase treated or untreated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80077-2-RR (Phospho-P53 (Ser392) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 80077-2-RR

    FC experiment of HeLa using 80077-2-RR

    1x10^6 λ phosphatase treated HeLa cells were intracellularly stained with 0.2 ug Phospho-P53 (Ser392) Recombinant Antibody (80077-2-RR, Clone:241575F6)(red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9)(blue) and PE-conjugated Goat Anti-Rabbit IgG. 1x10^6 untreated HeLa cells were intracellularly stained with 0.2 ug Phospho-P53 (Ser392) Recombinant Antibody (80077-2-RR, Clone:241575F6) and PE-conjugated Goat Anti-Rabbit IgG(black). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Phospho-P53 (Ser392) Recombinant antibody

Phospho-P53 (Ser392) Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 80077-2-RR

产品说明书

CloneNo. 241575F6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

p P53 Ser392, p P53 (Ser392), p P53, P53 (Ser392), Phospho P53

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inλ phosphatase treated HEK-293 cells
Positive FC (Intra) detected inλ phosphatase treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy P53 Ready-To-Use IHC Kit. P53 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80077-2-RR targets Phospho-P53 (Ser392) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tumor protein p53
别名 p P53 Ser392, p P53 (Ser392), p P53, P53 (Ser392), Phospho P53
计算分子量 44 kDa
观测分子量53 kDa
GenBank蛋白编号BC003596
基因名称 P53
Gene ID (NCBI) 7157
RRIDAB_3670464
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP04637
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

P53 is activated in response to alteration of normal cell homeostasis, including DNA damage, nutrient starvation, heat shock, virus infection, pH change, hypoxia, and oncogene activation. P53 maintains genetic stability by regulating different processes, such as cell-cycle arrest, DNA synthesis and repair, programmed cell death, and energy metabolism. In non-stressed conditions these proteins bind p53, ubiquitylate it and target it for degradation by the proteasome. In stressed conditions the function of the MdM2-MdM4 complex is blocked by phosphorylation, protein-binding events and/or enhanced degradation. (PMID: 19935675, PMID: 24379683)

实验方案

Product Specific Protocols
WB protocol for Phospho-P53 (Ser392) antibody 80077-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols