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验证数据展示

  • WB analysis using 80137-1-RR

    WB analysis using 80137-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80137-1-RR (MAP2K3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 80137-1-RR

    IF Staining of HeLa using 80137-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MAP2K3 antibody (80137-1-RR, Clone: 3O10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). DAPI (blue)

  • WB analysis of HepG2 using 80137-1-RR

    WB analysis of HepG2 using 80137-1-RR

    WB result of MAP2K3 antibody (80137-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MAP2K3 transfected HepG2 cells.

  • Featured Product
  • KD/KO Validated

MAP2K3 Recombinant antibody

MAP2K3 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 80137-1-RR

Print datasheet

CloneNo. 3O10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zebrafish

Applications

WB, IF/ICC, ELISA

Synonyms

MEK3, MAPKK 3, MAPK/ERK kinase 3, MAP kinase kinase 3, EC:2.7.12.2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inC6 cells, HepG2 cells, Jurkat cells, A431 cells, HT-1080 cells, zebrafish tissue, C2C12 cells, NIH/3T3 cells, HeLa cells, HEK-293 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80137-1-RR targets MAP2K3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat, zebrafish
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name mitogen-activated protein kinase kinase 3
Synonyms MEK3, MAPKK 3, MAPK/ERK kinase 3, MAP kinase kinase 3, EC:2.7.12.2
Calculated Molecular Weight 347 aa, 39 kDa
Observed Molecular Weight39 kDa
GenBank Accession NumberBC032478
Gene Symbol MAP2K3
Gene ID (NCBI) 5606
RRIDAB_2918868
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP46734
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAP2K3, also named MKK3, is a member of dual specificity kinase group and serves as a specific activator of p38 MAPK with MKK6. MKK3 can be phosphorylated by cytokines and environmental stress at sites Ser189 and Thr193 by MKKK proteins (MEKK 1-4). MKK3 plays a key role in cell differentiation, motility, division and death. It has been reported that MKK3 targeting may represent an effective and potentially safe therapeutic strategy to selectively kill cancer cells in colorectal cancer patients. (PMID: 31695024, 30770795, 26805700, 8622669)

实验方案

Product Specific Protocols
WB protocol for MAP2K3 antibody 80137-1-RRDownload protocol
IF protocol for MAP2K3 antibody 80137-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols