验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells, NIH/3T3 cells |
For other applications, we recommend the unconjugated version of this antibody, 80137-1-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-80137 targets MAP2K3 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC, FC (Intra) Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | mitogen-activated protein kinase kinase 3 |
| 别名 | MEK3, MAPKK 3, MAPK/ERK kinase 3, MAP kinase kinase 3, EC:2.7.12.2 |
| 计算分子量 | 347 aa, 39 kDa |
| 观测分子量 | 39 kDa |
| GenBank蛋白编号 | BC032478 |
| 基因名称 | MAP2K3 |
| Gene ID (NCBI) | 5606 |
| RRID | AB_3084797 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P46734 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MAP2K3, also named as MKK3, is a member of dual specificity kinase group and serves as a specific activator of p38 MAPK with MKK6. MKK3 can be phosphorylated by cytokines and environmental stress at sites Ser189 and Thr193 by MKKK proteins (MEKK 1-4). MKK3 plays a key role in cell differentiation, motility, division and death. It has been reported that MKK3 targeting may represent an effective and potentially safe therapeutic strategy to selectively kill cancer cells in colorectal cancer patients. (PMID: 31695024, 30770795, 26805700, 8622669)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 MAP2K3 antibody CL594-80137 | Download protocol |
| FC protocol for CL594 MAP2K3 antibody CL594-80137 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
