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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated MAP2K3 Recombinant antibody

MAP2K3 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3O10

Cat no : CL594-80137

Print datasheet

Synonyms

MEK3, MAPKK 3, MAPK/ERK kinase 3, MAP kinase kinase 3, EC:2.7.12.2

验证数据展示

  • IF Staining of HeLa using CL594-80137

    IF Staining of HeLa using CL594-80137

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 MAP2K3 antibody (CL594-80137, Clone: 3O10 ) at dilution of 1:200.

  • FC experiment of HeLa using CL594-80137

    FC experiment of HeLa using CL594-80137

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human MAP2K3 (CL594-80137, Clone:3O10) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells, NIH/3T3 cells
For other applications, we recommend the unconjugated version of this antibody, 80137-1-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-80137 targets MAP2K3 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name mitogen-activated protein kinase kinase 3
Synonyms MEK3, MAPKK 3, MAPK/ERK kinase 3, MAP kinase kinase 3, EC:2.7.12.2
Calculated Molecular Weight 347 aa, 39 kDa
Observed Molecular Weight39 kDa
GenBank Accession NumberBC032478
Gene Symbol MAP2K3
Gene ID (NCBI) 5606
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP46734
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAP2K3, also named as MKK3, is a member of dual specificity kinase group and serves as a specific activator of p38 MAPK with MKK6. MKK3 can be phosphorylated by cytokines and environmental stress at sites Ser189 and Thr193 by MKKK proteins (MEKK 1-4). MKK3 plays a key role in cell differentiation, motility, division and death. It has been reported that MKK3 targeting may represent an effective and potentially safe therapeutic strategy to selectively kill cancer cells in colorectal cancer patients. (PMID: 31695024, 30770795, 26805700, 8622669)

实验方案

Product Specific Protocols
IF protocol for CL594 MAP2K3 antibody CL594-80137Download protocol
FC protocol for CL594 MAP2K3 antibody CL594-80137Download protocol
Standard Protocols
Click here to view our Standard Protocols