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XRCC5 Recombinant antibody

XRCC5 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240301G6

Cat no : 80325-6-RR

Print datasheet

Synonyms

KU80, CTCBF, CTC85, CTC box-binding factor 85 kDa subunit, ATP-dependent DNA helicase II 80 kDa subunit

验证数据展示

  • WB analysis using 80325-6-RR

    WB analysis using 80325-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80325-6-RR (XRCC5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 80325-6-RR

    IF Staining of HepG2 using 80325-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using XRCC5 antibody (80325-6-RR, Clone: 240301G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of MCF-7 using 80325-6-RR

    FC experiment of MCF-7 using 80325-6-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug XRCC5 Recombinant antibody (80325-6-RR, Clone:240301G6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 80325-6-RR

    Affinity and Kinetic Characterization of 80325-6-RR

    Biolayer interferometry (BLl) kinetic assays of 80325-6-RR against Human XRCC5 were performed. The affinity constant is 4.59 nM.

经过测试的应用

Positive WB detected inHeLa cells, HepG cells, HEK-293 cells, A549 cells, K-562 cells
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy XRCC5 Ready-To-Use IHC Kit. XRCC5 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 80325-6-RR is XRCC5 Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen XRCC5 fusion protein Ag9454 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)
Synonyms KU80, CTCBF, CTC85, CTC box-binding factor 85 kDa subunit, ATP-dependent DNA helicase II 80 kDa subunit
Calculated Molecular Weight 732 aa, 83 kDa
Observed Molecular Weight80 kDa
GenBank Accession NumberBC019027
Gene Symbol XRCC5
Gene ID (NCBI) 7520
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP13010
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

There are at least two pathways for eukaryotes to repair DNA double-strand breaks: homologous recombination and nonhomologous end joining(NHEJ). The core NHEJ machinery includes XRCC4, DNA ligase IV and the DNA-dependent protein kinase complex, which consists of the DNA end-binding XRCC5/XRCC6 heterodimer and the catalytic subunit PRKDC. The heterdimer of XRCC5/XRCC6 enhanced teh affinity of the catalytic subunit PRKDC to DNA by 100-fold. Once the XRCC5/6 dimer association with NAA15, it can bind to the osteocalcin promoter and activate osteocalcin expression. The XRCC5/6 dimer acts as a negative regulator of transcription when together with APEX1. Some publised papers indicated that the MW of XRCC5 is 86kDa, while more papers suggested that XRCC5 is a 80kDa protein, as it was firstly introducted in publication.

实验方案

Product Specific Protocols
WB protocol for XRCC5 antibody 80325-6-RRDownload protocol
IF protocol for XRCC5 antibody 80325-6-RRDownload protocol
FC protocol for XRCC5 antibody 80325-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols