• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 80325-6-RR (same clone as 80325-6-PBS)

    WB analysis using 80325-6-RR (same clone as 80325-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80325-6-RR (XRCC5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80325-6-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 80325-6-RR (same clone as 80325-6-PBS)

    IHC staining of human colon cancer using 80325-6-RR (same clone as 80325-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80325-6-RR (XRCC5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80325-6-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with XRCC5 antibody included
  • IHC staining of human colon cancer using 80325-6-RR (same clone as 80325-6-PBS)

    IHC staining of human colon cancer using 80325-6-RR (same clone as 80325-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80325-6-RR (XRCC5 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80325-6-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with XRCC5 antibody included
  • IF Staining of HepG2 using 80325-6-RR (same clone as 80325-6-PBS)

    IF Staining of HepG2 using 80325-6-RR (same clone as 80325-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using XRCC5 antibody (80325-6-RR, Clone: 240301G6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80325-6-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 80325-6-RR (same clone as 80325-6-PBS)

    FC experiment of MCF-7 using 80325-6-RR (same clone as 80325-6-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug XRCC5 Recombinant antibody (80325-6-RR, Clone:240301G6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 80325-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80325-6-RR

    Affinity and Kinetic Characterization of 80325-6-RR

    Biolayer interferometry (BLl) kinetic assays of 80325-6-RR against Human XRCC5 were performed. The affinity constant is 4.59 nM.

XRCC5/Ku80 Recombinant antibody, PBS Only

XRCC5/Ku80 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 80325-6-PBS

产品说明书

CloneNo. 240301G6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

KU80, XRCC5, 240301G6, ATP-dependent DNA helicase 2 subunit 2, ATP-dependent DNA helicase II 80 kDa subunit

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy XRCC5 Ready-To-Use IHC Kit. XRCC5 primary antibody included.

产品信息

80325-6-PBS targets XRCC5/Ku80 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 XRCC5/Ku80 fusion protein Ag9454 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)
别名 KU80, XRCC5, 240301G6, ATP-dependent DNA helicase 2 subunit 2, ATP-dependent DNA helicase II 80 kDa subunit
计算分子量 732 aa, 83 kDa
观测分子量80 kDa
GenBank蛋白编号BC019027
基因名称 XRCC5
Gene ID (NCBI) 7520
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP13010
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

There are at least two pathways for eukaryotes to repair DNA double-strand breaks: homologous recombination and nonhomologous end joining(NHEJ). The core NHEJ machinery includes XRCC4, DNA ligase IV and the DNA-dependent protein kinase complex, which consists of the DNA end-binding XRCC5/XRCC6 heterodimer and the catalytic subunit PRKDC. The heterdimer of XRCC5/XRCC6 enhanced teh affinity of the catalytic subunit PRKDC to DNA by 100-fold. Once the XRCC5/6 dimer association with NAA15, it can bind to the osteocalcin promoter and activate osteocalcin expression. The XRCC5/6 dimer acts as a negative regulator of transcription when together with APEX1. Some publised papers indicated that the MW of XRCC5 is 86kDa, while more papers suggested that XRCC5 is a 80kDa protein, as it was firstly introducted in publication.