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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
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Compatible Species
0
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验证数据展示

  • WB analysis using 80325-6-RR (same clone as 80325-6-PBS)

    WB analysis using 80325-6-RR (same clone as 80325-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80325-6-RR (XRCC5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80325-6-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 80325-6-RR (same clone as 80325-6-PBS)

    IHC staining of human colon cancer using 80325-6-RR (same clone as 80325-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80325-6-RR (XRCC5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80325-6-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with XRCC5 antibody included
  • IHC staining of human colon cancer using 80325-6-RR (same clone as 80325-6-PBS)

    IHC staining of human colon cancer using 80325-6-RR (same clone as 80325-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80325-6-RR (XRCC5 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80325-6-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with XRCC5 antibody included
  • IF Staining of HepG2 using 80325-6-RR (same clone as 80325-6-PBS)

    IF Staining of HepG2 using 80325-6-RR (same clone as 80325-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using XRCC5 antibody (80325-6-RR, Clone: 240301G6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80325-6-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 80325-6-RR (same clone as 80325-6-PBS)

    FC experiment of MCF-7 using 80325-6-RR (same clone as 80325-6-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug XRCC5 Recombinant antibody (80325-6-RR, Clone:240301G6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 80325-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80325-6-RR

    Affinity and Kinetic Characterization of 80325-6-RR

    Biolayer interferometry (BLl) kinetic assays of 80325-6-RR against Human XRCC5 were performed. The affinity constant is 4.59 nM.

XRCC5 Recombinant antibody, PBS Only

XRCC5 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 80325-6-PBS

Print datasheet

CloneNo. 240301G6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

KU80, CTCBF, CTC85, CTC box-binding factor 85 kDa subunit, ATP-dependent DNA helicase II 80 kDa subunit

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy XRCC5 Ready-To-Use IHC Kit. XRCC5 primary antibody included.

产品信息

80325-6-PBS targets XRCC5 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen XRCC5 fusion protein Ag9454 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)
Synonyms KU80, CTCBF, CTC85, CTC box-binding factor 85 kDa subunit, ATP-dependent DNA helicase II 80 kDa subunit
Calculated Molecular Weight 732 aa, 83 kDa
Observed Molecular Weight80 kDa
GenBank Accession NumberBC019027
Gene Symbol XRCC5
Gene ID (NCBI) 7520
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP13010
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

There are at least two pathways for eukaryotes to repair DNA double-strand breaks: homologous recombination and nonhomologous end joining(NHEJ). The core NHEJ machinery includes XRCC4, DNA ligase IV and the DNA-dependent protein kinase complex, which consists of the DNA end-binding XRCC5/XRCC6 heterodimer and the catalytic subunit PRKDC. The heterdimer of XRCC5/XRCC6 enhanced teh affinity of the catalytic subunit PRKDC to DNA by 100-fold. Once the XRCC5/6 dimer association with NAA15, it can bind to the osteocalcin promoter and activate osteocalcin expression. The XRCC5/6 dimer acts as a negative regulator of transcription when together with APEX1. Some publised papers indicated that the MW of XRCC5 is 86kDa, while more papers suggested that XRCC5 is a 80kDa protein, as it was firstly introducted in publication.