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XRCC5 Recombinant antibody, PBS Only

XRCC5 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240301G6

Cat no : 80325-6-PBS

Print datasheet

Synonyms

KU80, CTCBF, CTC85, CTC box-binding factor 85 kDa subunit, ATP-dependent DNA helicase II 80 kDa subunit

验证数据展示

  • WB analysis using 80325-6-RR (same clone as 80325-6-PBS)

    WB analysis using 80325-6-RR (same clone as 80325-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80325-6-RR (XRCC5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80325-6-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 80325-6-RR (same clone as 80325-6-PBS)

    IF Staining of HepG2 using 80325-6-RR (same clone as 80325-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using XRCC5 antibody (80325-6-RR, Clone: 240301G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80325-6-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 80325-6-RR (same clone as 80325-6-PBS)

    FC experiment of MCF-7 using 80325-6-RR (same clone as 80325-6-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug XRCC5 Recombinant antibody (80325-6-RR, Clone:240301G6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 80325-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80325-6-RR

    Affinity and Kinetic Characterization of 80325-6-RR

    Biolayer interferometry (BLl) kinetic assays of 80325-6-RR against Human XRCC5 were performed. The affinity constant is 4.59 nM.

Planning an IHC experiment? We recommend our IHCeasy XRCC5 Ready-To-Use IHC Kit. XRCC5 primary antibody included.

产品信息

The immunogen of 80325-6-PBS is XRCC5 Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen XRCC5 fusion protein Ag9454 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)
Synonyms KU80, CTCBF, CTC85, CTC box-binding factor 85 kDa subunit, ATP-dependent DNA helicase II 80 kDa subunit
Calculated Molecular Weight 732 aa, 83 kDa
GenBank Accession NumberBC019027
Gene Symbol XRCC5
Gene ID (NCBI) 7520
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP13010
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C