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MCP-1/CCL2 Recombinant antibody, PBS Only (Capture)

MCP-1/CCL2 Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241165B11

Cat no : 80847-4-PBS

Print datasheet

Synonyms

CCL2, MCP1, MCP-1, CCL-2, CCL 2

验证数据展示

  • Sandwich ELISA standard curve of MP00938-1

    Sandwich ELISA standard curve of MP00938-1

    Sandwich ELISA standard curve of MP00938-1, Human MCP-1 Recombinant Matched Antibody Pair - PBS only. 80847-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg31375. 80847-7-PBS was HRP conjugated as the detection antibody. Range: 7.8-500 pg/mL

  • Sample test of MP00938-1

    Sample test of MP00938-1

    Urine of eight individual healthy human donors was measured. The human MCP-1 concentration of detected samples was determined to be 385.1 pg/mL with a range of 106.1 - 999.8 pg/mL.

  • Sample test of MP00938-1

    Sample test of MP00938-1

    Human peripheral blood mononuclear cells (1 x 10^6 cells/mL) were cultured in RPMI-1640 supplemented with 10% fetal bovine serum, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. The cells were unstimulated or stimulated with 10 μg/mL PHA. Aliquots of the cell culture supernates were removed on days 3 and 5 and assayed for levels of human MCP-1.

  • Sample test of MP00938-1

    Sample test of MP00938-1

    Serum of sixteen individual healthy human donors was measured. The human MCP-1 concentration of detected samples was determined to be 595.9 pg/mL with a range of 193.4 - 1,105.3 pg/mL.

  • Cytometric bead array standard curve of MP00938-1

    Cytometric bead array standard curve of MP00938-1

    Cytometric bead array standard curve of MP00938-1, MCP-1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 80847-4-PBS. Detection antibody: 80847-7-PBS. Standard: Eg31375. Range: 0.313-20 ng/mL.

产品信息

80847-4-PBS targets MCP-1/CCL2 as part of a matched antibody pair:

MP00938-1: 80847-4-PBS capture and 80847-7-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name chemokine (C-C motif) ligand 2
Synonyms CCL2, MCP1, MCP-1, CCL-2, CCL 2
Calculated Molecular Weight 99 aa, 11 kDa
GenBank Accession NumberBC009716
Gene Symbol MCP-1/CCL2
Gene ID (NCBI) 6347
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP13500
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C