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验证数据展示

  • WB analysis using 80876-2-RR

    WB analysis using 80876-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80876-2-RR (Ag11509 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse brain using 80876-2-RR

    IF Staining of mouse brain using 80876-2-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using YTHDF1 antibody (80876-2-RR, Clone: 241445B10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HEK-293 using 80876-2-RR

    FC experiment of HEK-293 using 80876-2-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug YTHDF1 Recombinant antibody (80876-2-RR, Clone:241445B10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 80876-2-RR

    Affinity and Kinetic Characterization of 80876-2-RR

    Biolayer interferometry (BLl) kinetic assays of 80876-2-RR against Human YTHDF1 were performed. The affinity constant is 7.23 nM.

YTHDF1 Recombinant antibody

YTHDF1 Recombinant Antibody for WB, IF-P, FC (Intra), ELISA
Cat No. 80876-2-RR

产品说明书

CloneNo. 241445B10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF-P, FC (Intra), ELISA

别名

YTH domain-containing family protein 1, DF1, DACA-1, C20orf21, 241445B10

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, HepG2 cells, K-562 cells, NIH/3T3 cells, HSC-T6 cells
Positive IF-P detected inmouse brain tissue
Positive FC (Intra) detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy YTHDF1 Ready-To-Use IHC Kit. YTHDF1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80876-2-RR targets YTHDF1 in WB, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF-P, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 YTHDF1 fusion protein Ag11509 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 YTH domain family, member 1
别名 YTH domain-containing family protein 1, DF1, DACA-1, C20orf21, 241445B10
计算分子量 559 aa, 61 kDa
观测分子量60-65 kDa
GenBank蛋白编号BC050284
基因名称 YTHDF1
Gene ID (NCBI) 54915
RRIDAB_3670491
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BYJ9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for YTHDF1 antibody 80876-2-RRDownload protocol
IF protocol for YTHDF1 antibody 80876-2-RRDownload protocol
FC protocol for YTHDF1 antibody 80876-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols