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Host
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Conjugate
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验证数据展示

  • WB analysis using 80876-2-RR (same clone as 80876-2-PBS)

    WB analysis using 80876-2-RR (same clone as 80876-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80876-2-RR (Ag11509 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80876-2-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 80876-2-RR (same clone as 80876-2-PBS)

    IF Staining of mouse brain using 80876-2-RR (same clone as 80876-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using YTHDF1 antibody (80876-2-RR, Clone: 241445B10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80876-2-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 80876-2-RR (same clone as 80876-2-PBS)

    FC experiment of HEK-293 using 80876-2-RR (same clone as 80876-2-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug YTHDF1 Recombinant antibody (80876-2-RR, Clone:241445B10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 80876-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01146-3

    Cytometric bead array standard curve of MP01146-3

    Cytometric bead array standard curve of MP01146-3, YTHDF1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 80876-4-PBS. Detection antibody: 80876-2-PBS. Standard: Ag11509. Range: 0.469-60 ng/mL.

  • Affinity and Kinetic Characterization of 80876-2-RR

    Affinity and Kinetic Characterization of 80876-2-RR

    Biolayer interferometry (BLl) kinetic assays of 80876-2-RR against Human YTHDF1 were performed. The affinity constant is 7.23 nM.

YTHDF1 Recombinant antibody, PBS Only (Detector)

YTHDF1 Uni-rAbTM Recombinant Antibody for WB, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 80876-2-PBS

产品说明书

CloneNo. 241445B10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA

别名

YTH domain-containing family protein 1, DF1, DACA-1, C20orf21, 241445B10

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy YTHDF1 Ready-To-Use IHC Kit. YTHDF1 primary antibody included.

产品信息

80876-2-PBS targets YTHDF1 as part of a matched antibody pair:

MP01146-3: 80876-4-PBS capture and 80876-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 YTHDF1 fusion protein Ag11509 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 YTH domain family, member 1
别名 YTH domain-containing family protein 1, DF1, DACA-1, C20orf21, 241445B10
计算分子量 559 aa, 61 kDa
观测分子量60-65 kDa
GenBank蛋白编号BC050284
基因名称 YTHDF1
Gene ID (NCBI) 54915
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BYJ9
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C