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CP110 Recombinant antibody

CP110 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

242016E10

Cat no : 80965-5-RR

Print datasheet

Synonyms

CP110 protein, CP 110, Cep110, Centriolar coiled-coil protein of 110 kDa, CCP110

验证数据展示

  • WB analysis using 80965-5-RR

    WB analysis using 80965-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80965-5-RR (CP110 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 80965-5-RR

    IHC staining of mouse testis using 80965-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 80965-5-RR (CP110 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CP110 antibody included
  • IF Staining of HepG2 using 80965-5-RR

    IF Staining of HepG2 using 80965-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CP110 antibody (80965-5-RR, Clone: 242016E10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 80965-5-RR

    Affinity and Kinetic Characterization of 80965-5-RR

    Biolayer interferometry (BLl) kinetic assays of 80965-5-RR against Human CP110 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHEK-293T cells, Jurkat cells, MOLT-4 cells, HCT 116 cells, HeLa cells, K-562 cells, U2OS cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CP110 Ready-To-Use IHC Kit. CP110 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80965-5-RR targets CP110 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen CP110 fusion protein Ag3489 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CP110 protein
Synonyms CP110 protein, CP 110, Cep110, Centriolar coiled-coil protein of 110 kDa, CCP110
Calculated Molecular Weight 991 aa, 111 kDa
Observed Molecular Weight 113 kDa
GenBank Accession NumberBC036654
Gene Symbol CP110
Gene ID (NCBI) 9738
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO43303
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CP110, also named as CCP110 and KIAA0419, is an 110kDa protein. This gene is different to the gene CEP110 (geneID:11064; CNTRL). CP110 is a centriolar protein that positively regulates centrioleduplication while restricting centriole elongation and ciliogenesis. And it acts as a key negative regulator of ciliogenesis in collaboration with CEP97 by capping the mother centriole thereby preventing cilia formation.

实验方案

Product Specific Protocols
WB protocol for CP110 antibody 80965-5-RRDownload protocol
IHC protocol for CP110 antibody 80965-5-RRDownload protocol
IF protocol for CP110 antibody 80965-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols