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CP110 Recombinant antibody, PBS Only (Detector)

CP110 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

242016E10

Cat no : 80965-5-PBS

Print datasheet

Synonyms

CP110 protein, CP 110, Cep110, Centriolar coiled-coil protein of 110 kDa, CCP110

验证数据展示

  • WB analysis using 80965-5-RR (same clone as 80965-5-PBS)

    WB analysis using 80965-5-RR (same clone as 80965-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80965-5-RR (CP110 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80965-5-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01447-3

    Sandwich ELISA standard curve of MP01447-3

    Sandwich ELISA standard curve of MP01447-3, Human CP110 Recombinant Matched Antibody Pair - PBS only. 80965-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3489. 80965-5-PBS was HRP conjugated as the detection antibody. Range: 0.625-40 ng/mL

  • Sample test of MP01447-3

    Sample test of MP01447-3

    The mean CP110 concentration was determined to be 31.64 ng/mL in Jurkat cell extract based on a 4.2 mg/mL extract load and 9.42 ng/mL in HEK-293 cell extract based on a 1.2 mg/mL extract load.

  • IHC staining of mouse testis using 80965-5-RR (same clone as 80965-5-PBS)

    IHC staining of mouse testis using 80965-5-RR (same clone as 80965-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 80965-5-RR (CP110 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80965-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CP110 antibody included
  • IF Staining of HepG2 using 80965-5-RR (same clone as 80965-5-PBS)

    IF Staining of HepG2 using 80965-5-RR (same clone as 80965-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CP110 antibody (80965-5-RR, Clone: 242016E10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80965-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80965-5-RR

    Affinity and Kinetic Characterization of 80965-5-RR

    Biolayer interferometry (BLl) kinetic assays of 80965-5-RR against Human CP110 were performed. The affinity constant is below 1 pM.

Planning an IHC experiment? We recommend our IHCeasy CP110 Ready-To-Use IHC Kit. CP110 primary antibody included.

产品信息

80965-5-PBS targets CP110 as part of a matched antibody pair:

MP01447-3: 80965-4-PBS capture and 80965-5-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen CP110 fusion protein Ag3489 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CP110 protein
Synonyms CP110 protein, CP 110, Cep110, Centriolar coiled-coil protein of 110 kDa, CCP110
Calculated Molecular Weight 991 aa, 111 kDa
Observed Molecular Weight 113 kDa
GenBank Accession NumberBC036654
Gene Symbol CP110
Gene ID (NCBI) 9738
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO43303
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CP110, also named as CCP110 and KIAA0419, is an 110kDa protein. This gene is different to the gene CEP110 (geneID:11064; CNTRL). CP110 is a centriolar protein that positively regulates centrioleduplication while restricting centriole elongation and ciliogenesis. And it acts as a key negative regulator of ciliogenesis in collaboration with CEP97 by capping the mother centriole thereby preventing cilia formation.