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  • Featured Product
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DHFR Recombinant antibody

DHFR Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

7P5

Cat no : 81021-1-RR

Print datasheet

Synonyms

EC:1.5.1.3, DHFRP1, 7P5

验证数据展示

  • WB analysis using 81021-1-RR

    WB analysis using 81021-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81021-1-RR (DHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 81021-1-RR

    WB analysis of HeLa using 81021-1-RR

    WB result of DHFR antibody (81021-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DHFR transfected HeLa cells.

  • WB analysis using 81021-1-RR

    WB analysis using 81021-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81021-1-RR (DHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 81021-1-RR

    IHC staining of human liver cancer using 81021-1-RR

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81021-1-RR (DHFR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 81021-1-RR

    IHC staining of human liver cancer using 81021-1-RR

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81021-1-RR (DHFR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 81021-1-RR

    Affinity and Kinetic Characterization of 81021-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81021-1-RR against Human DHFR were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inK-562 cells, HeLa cells, Jurkat cells, HEK-293 cells, MOLT-4 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81021-1-RR targets DHFR in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen DHFR fusion protein Ag7331 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name dihydrofolate reductase
Synonyms EC:1.5.1.3, DHFRP1, 7P5
Calculated Molecular Weight 21 kDa
Observed Molecular Weight21 kDa
GenBank Accession NumberBC000192
Gene Symbol DHFR
Gene ID (NCBI) 1719
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP00374
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DHFR (dihydrofolate reductase) catalyzes the subsequent NADPH-dependent reduction of the H2folate produced by TS to regenerate the tetrahydrofolate (H4folate) necessary for continued dTMP synthesis and transfer of 1-carbon units (PMID:3461439). It belongs to the dihydrofolate reductase family and can exist as a homodimer (PMID:2248959). DHFR is widely expressed in fetal and adult human brain and whole blood, expression is higher in adult brain than fetal brain (PMID:21310276).

实验方案

Product Specific Protocols
WB protocol for DHFR antibody 81021-1-RRDownload protocol
IHC protocol for DHFR antibody 81021-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols