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Species Reactivity
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0
Conjugate
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验证数据展示

  • WB analysis using 81021-1-RR (same clone as 81021-1-PBS)

    WB analysis using 81021-1-RR (same clone as 81021-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81021-1-RR (DHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81021-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 81021-1-RR (same clone as 81021-1-PBS)

    WB analysis of HeLa using 81021-1-RR (same clone as 81021-1-PBS)

    WB result of DHFR antibody (81021-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DHFR transfected HeLa cells. This data was developed using the same antibody clone with 81021-1-PBS in a different storage buffer formulation.

  • WB analysis using 81021-1-RR (same clone as 81021-1-PBS)

    WB analysis using 81021-1-RR (same clone as 81021-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81021-1-RR (DHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81021-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 81021-1-RR (same clone as 81021-1-PBS)

    IHC staining of human liver cancer using 81021-1-RR (same clone as 81021-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81021-1-RR (DHFR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81021-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 81021-1-RR (same clone as 81021-1-PBS)

    IHC staining of human liver cancer using 81021-1-RR (same clone as 81021-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81021-1-RR (DHFR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81021-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81021-1-RR

    Affinity and Kinetic Characterization of 81021-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81021-1-RR against Human DHFR were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

DHFR Recombinant antibody, PBS Only

DHFR Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 81021-1-PBS

产品说明书

CloneNo. 7P5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

EC:1.5.1.3, DHFRP1, 7P5

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

81021-1-PBS targets DHFR in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原 DHFR fusion protein Ag7331 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 dihydrofolate reductase
别名 EC:1.5.1.3, DHFRP1, 7P5
计算分子量 21 kDa
观测分子量21 kDa
GenBank蛋白编号BC000192
基因名称 DHFR
Gene ID (NCBI) 1719
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP00374
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DHFR (dihydrofolate reductase) catalyzes the subsequent NADPH-dependent reduction of the H2folate produced by TS to regenerate the tetrahydrofolate (H4folate) necessary for continued dTMP synthesis and transfer of 1-carbon units (PMID:3461439). It belongs to the dihydrofolate reductase family and can exist as a homodimer (PMID:2248959). DHFR is widely expressed in fetal and adult human brain and whole blood, expression is higher in adult brain than fetal brain (PMID:21310276).