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  • Featured Product
  • KD/KO Validated

DHFR Recombinant antibody, PBS Only

DHFR Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

7P5

Cat no : 81021-1-PBS

Print datasheet

Synonyms

EC:1.5.1.3, DHFRP1, 7P5

验证数据展示

  • WB analysis using 81021-1-RR (same clone as 81021-1-PBS)

    WB analysis using 81021-1-RR (same clone as 81021-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81021-1-RR (DHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81021-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 81021-1-RR (same clone as 81021-1-PBS)

    WB analysis of HeLa using 81021-1-RR (same clone as 81021-1-PBS)

    WB result of DHFR antibody (81021-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DHFR transfected HeLa cells. This data was developed using the same antibody clone with 81021-1-PBS in a different storage buffer formulation.

  • WB analysis using 81021-1-RR (same clone as 81021-1-PBS)

    WB analysis using 81021-1-RR (same clone as 81021-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81021-1-RR (DHFR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81021-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 81021-1-RR (same clone as 81021-1-PBS)

    IHC staining of human liver cancer using 81021-1-RR (same clone as 81021-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81021-1-RR (DHFR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81021-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 81021-1-RR (same clone as 81021-1-PBS)

    IHC staining of human liver cancer using 81021-1-RR (same clone as 81021-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81021-1-RR (DHFR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81021-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81021-1-RR

    Affinity and Kinetic Characterization of 81021-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81021-1-RR against Human DHFR were performed. The affinity constant is below 1 pM.

产品信息

81021-1-PBS targets DHFR in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen DHFR fusion protein Ag7331 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name dihydrofolate reductase
Synonyms EC:1.5.1.3, DHFRP1, 7P5
Calculated Molecular Weight 21 kDa
Observed Molecular Weight21 kDa
GenBank Accession NumberBC000192
Gene Symbol DHFR
Gene ID (NCBI) 1719
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP00374
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DHFR (dihydrofolate reductase) catalyzes the subsequent NADPH-dependent reduction of the H2folate produced by TS to regenerate the tetrahydrofolate (H4folate) necessary for continued dTMP synthesis and transfer of 1-carbon units (PMID:3461439). It belongs to the dihydrofolate reductase family and can exist as a homodimer (PMID:2248959). DHFR is widely expressed in fetal and adult human brain and whole blood, expression is higher in adult brain than fetal brain (PMID:21310276).