TDP-43 (C-terminal) Recombinant antibody
TDP-43 (C-terminal) Recombinant Antibody for WB, IF/ICC, FC, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse, Rat and More (2)
Applications
WB, IF/ICC, FC, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
5D17
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, NIH/3T3 cells, HSC-T6 cells |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
Positive FC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF) | IF : 1:2500-1:10000 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
81350-1-RR targets TDP-43 (C-terminal) as part of a matched antibody pair:
MP00589-1: 80007-1-RR capture and 81350-1-RR detection (validated in Cytometric bead array)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
Tested Applications | WB, IF/ICC, FC, IHC, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | Human, Mouse, Rat |
Cited Reactivity | human, mouse |
Immunogen | fusion protein 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | TAR DNA binding protein |
Synonyms | ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43, TDP-43 |
Calculated Molecular Weight | 43 kDa |
Observed Molecular Weight | 43 kDa |
GenBank Accession Number | BC001487 |
Gene Symbol | TARDBP |
Gene ID (NCBI) | 23435 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q13148 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for TDP-43 (C-terminal) antibody 81350-1-RR | Download protocol |
IHC protocol for TDP-43 (C-terminal) antibody 81350-1-RR | Download protocol |
IF protocol for TDP-43 (C-terminal) antibody 81350-1-RR | Download protocol |
FC protocol for TDP-43 (C-terminal) antibody 81350-1-RR | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|