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TDP-43 (C-terminal) Recombinant antibody, PBS Only (Detector)

TDP-43 (C-terminal) Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5D17

Cat no : 81350-1-PBS

Print datasheet

Synonyms

TARDBP, TDP 43, TDP43, TDP-43, TAR DNA-binding protein 43

验证数据展示

  • WB analysis using 81350-1-RR (same clone as 81350-1-PBS)

    WB analysis using 81350-1-RR (same clone as 81350-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81350-1-RR (TDP-43 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81350-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 81350-1-RR (same clone as 81350-1-PBS)

    IF Staining of HeLa using 81350-1-RR (same clone as 81350-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TDP-43 (C-terminal) antibody (81350-1-RR, Clone: 5D17 ) at dilution of 1:5000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81350-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 81350-1-RR (same clone as 81350-1-PBS)

    IHC staining of mouse brain using 81350-1-RR (same clone as 81350-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81350-1-RR (TDP-43 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81350-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TDP-43 antibody included
  • IHC staining of mouse brain using 81350-1-RR (same clone as 81350-1-PBS)

    IHC staining of mouse brain using 81350-1-RR (same clone as 81350-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81350-1-RR (TDP-43 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81350-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TDP-43 antibody included
  • FC experiment of HeLa using 81350-1-RR (same clone as 81350-1-PBS)

    FC experiment of HeLa using 81350-1-RR (same clone as 81350-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TDP-43 (C-terminal) (81350-1-RR, Clone:5D17) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 81350-1-PBS in a different storage buffer formulation.

  • Cytometric bead array in cell lysate using MP00589-1

    Cytometric bead array in cell lysate using MP00589-1

    Cytometric bead array in cell lysate using MP00589-1, phospho(409/410)-TDP43 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 80007-1-PBS. Detection antibody: 81350-1-PBS. Cell lysate(30μg/well): Non-treated HeLa and Calyculin A treated HeLa cells were lysed in 8M urea buffer and diluted in assay buffer. Non-related target Tubulin-Alpha Recombinant Matched Antibody Pair (MP00551-1) was served as control.

  • Affinity and Kinetic Characterization of 81350-1-RR

    Affinity and Kinetic Characterization of 81350-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81350-1-RR against Human TDP-43 (C-terminal) were performed. The affinity constant is below 1 pM.

Planning an IHC experiment? We recommend our IHCeasy TDP-43 Ready-To-Use IHC Kit. TDP-43 primary antibody included.

产品信息

81350-1-PBS targets TDP-43 (C-terminal) as part of a matched antibody pair:

MP00589-1: 80007-1-PBS capture and 81350-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen fusion protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name TAR DNA binding protein
Synonyms TARDBP, TDP 43, TDP43, TDP-43, TAR DNA-binding protein 43
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC001487
Gene Symbol TDP-43
Gene ID (NCBI) 23435
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13148
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene. In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS).