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Phospho-TDP43 (Ser409/410) Recombinant antibody, PBS Only (Capture)

Phospho-TDP43 (Ser409/410) Recombinant Antibody for WB, FC, IHC, Indirect ELISA, Cytometric bead array

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, FC, IHC, Indirect ELISA, Cytometric bead array

Conjugate

Unconjugated

CloneNo.

6M10

Cat No : 80007-1-PBS

Print datasheet

Synonyms

ALS10, Phospho-TDP43 (Ser409/410), TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43, tdp-43

验证数据展示

  • IHC staining of frontal cortex of FTLD-TDP type B case using 80007-1-RR (same clone as 80007-1-PBS)

    IHC staining of frontal cortex of FTLD-TDP type B case using 80007-1-RR (same clone as 80007-1-PBS)

    IHC results of Phospho-TDP43 (Ser409/410) rabbit recombinant antibody (80007-1-RR, 1000) with the frontal cortex from FTLD-TDP type B patients. IHC experiment was done with Ventana automatic staining system and Optiview DAB detection kit with heat-induced epitope retrieval (boiling for 32 min in Tris-EDTA based solution CC1 buffer, Ventana). Fig from the lab of Dr. Neumann. This data was developed using the same antibody clone with 80007-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TDP-43 antibody included
  • IHC staining of human brain using 80007-1-RR (same clone as 80007-1-PBS)

    IHC staining of human brain using 80007-1-RR (same clone as 80007-1-PBS)

    It's human subiculum (high-mag) and temporal cortex (medium-mag) from subject with limbic-predominant age-related TDP-43 encephalopathy neuropathologic change (LATE-NC). Staining provided by Pete Nelson and Ela Patel, U. Kentucky AD Research Center Neuropathology Core. This data was developed using the same antibody clone with 80007-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TDP-43 antibody included
  • WB analysis of HeLa using 80007-1-RR (same clone as 80007-1-PBS)

    WB analysis of HeLa using 80007-1-RR (same clone as 80007-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 80007-1-RR (Phospho-TDP43 (Ser409/410) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80007-1-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 80007-1-RR (same clone as 80007-1-PBS)

    FC experiment of Jurkat using 80007-1-RR (same clone as 80007-1-PBS)

    1X10^6 Jurkat cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.5 ug Anti-Human Phospho-TDP43 (Ser409/410) (80007-1-RR, Clone:6M10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 , or 0.5 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 80007-1-PBS in a different storage buffer formulation.

  • Cytometric bead array in cell lysate using MP00589-1

    Cytometric bead array in cell lysate using MP00589-1

    Cytometric bead array in cell lysate using MP00589-1, phospho(409/410)-TDP43 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 80007-1-PBS. Detection antibody: 81350-1-PBS. Cell lysate(30μg/well): Non-treated HeLa and Calyculin A treated HeLa cells were lysed in 8M urea buffer and diluted in assay buffer. Non-related target Tubulin-Alpha Recombinant Matched Antibody Pair (MP00551-1) was served as control.

  • Cytometric bead array in cell lysate using MP00589-2

    Cytometric bead array in cell lysate using MP00589-2

    Cytometric bead array in cell lysate using MP00589-2, phospho(409/410)-TDP43 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 80007-1-PBS. Detection antibody: 80002-1-PBS. Cell lysate(30μg/well): Non-treated HeLa and Calyculin A treated HeLa cells were lysed in 8M urea buffer and diluted in assay buffer. Non-related target Tubulin-Alpha Recombinant Matched Antibody Pair (MP00551-1) was served as control.

Planning an IHC experiment? We recommend our IHCeasy TDP-43 Ready-To-Use IHC Kit. TDP-43 primary antibody included.

产品信息

80007-1-PBS targets Phospho-TDP43 (Ser409/410) as part of a matched antibody pair:

MP00589-1: 80007-1-PBS capture and 81350-1-PBS detection (validated in N/A)

MP00589-2: 80007-1-PBS capture and 80002-1-PBS detection (validated in N/A)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, FC, IHC, Indirect ELISA, Cytometric bead array Application Description
Tested Reactivity Human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name TAR DNA binding protein
Synonyms ALS10, Phospho-TDP43 (Ser409/410), TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43, tdp-43
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberNM_007375
Gene Symbol TARDBP
Gene ID (NCBI) 23435
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13148
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176). 80007-1-RR is a recombinant rabbit monoclonal antibody recognizing TDP-43 only when phosphorylated at 409/410. Immunohistochemical analyses using this antibody only stain the insoluble inclusions in pathologic tissues without normal diffuse nuclear staining.