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  • Featured Product
  • KD/KO Validated

TDP-43 Recombinant antibody, PBS Only (Detector)

TDP-43 Recombinant Antibody for WB, IP, IF, FC, Indirect ELISA, Cytometric bead array

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IP, IF, FC, Indirect ELISA, Cytometric bead array

Conjugate

Unconjugated

CloneNo.

16A22

Cat No : 80002-1-PBS

Print datasheet

Synonyms

ALS10, TAR DNA binding protein 43, TARDBP, tdp 43, TDP43, TDP-43

验证数据展示

  • WB analysis using 80002-1-RR (same clone as 80002-1-PBS)

    WB analysis using 80002-1-RR (same clone as 80002-1-PBS)

    Various cell lysates were subjected to SDS PAGE followed by western blot with 80002-1-RR (TDP-43 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80002-1-PBS in a different storage buffer formulation.

  • WB analysis of HAP1 using 80002-1-RR (same clone as 80002-1-PBS)

    WB analysis of HAP1 using 80002-1-RR (same clone as 80002-1-PBS)

    HAP1 (WT and TARDBP KO) lysates prepared with NP-40 buffer, 50 μg protein loaded. 80002-1-RR incubated at 1:1000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 80002-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 80002-1-RR (same clone as 80002-1-PBS)

    WB analysis of HeLa using 80002-1-RR (same clone as 80002-1-PBS)

    WB result of TDP-43 antibody (80002-1-RR; 1:80000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TDP-43 transfected HeLa cells. This data was developed using the same antibody clone with 80002-1-PBS in a different storage buffer formulation.

  • IP experiment of HAP1 using 80002-1-RR (same clone as 80002-1-PBS)

    IP experiment of HAP1 using 80002-1-RR (same clone as 80002-1-PBS)

    HAP1 lysates prepared and IP of TARDBP performed using 2.0 μg of 80002-1-RR coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 80002-1-PBS in a different storage buffer formulation.

  • IF Staining of rat brain using 80002-1-RR (same clone as 80002-1-PBS)

    IF Staining of rat brain using 80002-1-RR (same clone as 80002-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 80002-1-RR (TDP-43 antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80002-1-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 80002-1-RR (same clone as 80002-1-PBS)

    IF Staining of SH-SY5Y using 80002-1-RR (same clone as 80002-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using TDP-43 antibody (80002-1-RR, Clone: 16A22 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Red: staining with CoraLite555-Phalloidin. This data was developed using the same antibody clone with 80002-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 80002-1-RR (same clone as 80002-1-PBS)

    IF Staining of HeLa using 80002-1-RR (same clone as 80002-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TDP-43 antibody (80002-1-RR, Clone: 16A22 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80002-1-PBS in a different storage buffer formulation.

  • IF Staining of HAP1 using 80002-1-RR (same clone as 80002-1-PBS)

    IF Staining of HAP1 using 80002-1-RR (same clone as 80002-1-PBS)

    HAP1 WT cells (yellow outline) and TARDBP KO cells (red outline) labelled with a green or a far red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 80002-1-RR at 1:200 plus DAPI. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 80002-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 80002-1-RR (same clone as 80002-1-PBS)

    FC experiment of HeLa using 80002-1-RR (same clone as 80002-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TDP-43 (for IF/FC) (80002-1-RR, Clone:16A22) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 80002-1-PBS in a different storage buffer formulation.

  • Cytometric bead array in cell lysate using MP00589-2

    Cytometric bead array in cell lysate using MP00589-2

    Cytometric bead array in cell lysate using MP00589-2, phospho(409/410)-TDP43 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 80007-1-PBS. Detection antibody: 80002-1-PBS. Cell lysate(30μg/well): Non-treated HeLa and Calyculin A treated HeLa cells were lysed in 8M urea buffer and diluted in assay buffer. Non-related target Tubulin-Alpha Recombinant Matched Antibody Pair (MP00551-1) was served as control.

Planning an IHC experiment? We recommend our IHCeasy TDP-43 Ready-To-Use IHC Kit. TDP-43 primary antibody included.

产品信息

80002-1-PBS targets TDP-43 as part of a matched antibody pair:

MP00589-2: 80007-1-PBS capture and 80002-1-PBS detection (validated in N/A)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IP, IF, FC, Indirect ELISA, Cytometric bead array Application Description
Tested Reactivity Human, mouse, rat
Immunogen TDP-43 fusion protein Ag1231 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name TAR DNA binding protein
Synonyms ALS10, TAR DNA binding protein 43, TARDBP, tdp 43, TDP43, TDP-43
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC001487
Gene Symbol TARDBP
Gene ID (NCBI) 23435
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13148
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene. In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS). Proteintech's 80002-1-RR is a rabbit recombinant TDP-43 antibody recognizing N-terminal TDP-43. It recognizes the intact 43 kDa protein as well as all posttranslationally modified and truncated forms in multiple applications. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659, 19823856, 21666678, 22193176) Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (PMID: 29581274)

80002-1-RR antibody works well in IF experiment.