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Phospho-TDP43 (Ser409/410) Recombinant antibody

Phospho-TDP43 (Ser409/410) Recombinant Antibody for WB, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, FC, IHC, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

6M10

Cat No : 80007-1-RR

Print datasheet

Synonyms

ALS10, Phospho-TDP43 (Ser409/410), TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43, tdp-43

验证数据展示

  • IHC staining of frontal cortex of FTLD-TDP type B case using 80007-1-RR

    IHC staining of frontal cortex of FTLD-TDP type B case using 80007-1-RR

    IHC results of Phospho-TDP43 (Ser409/410) rabbit recombinant antibody (80007-1-RR, 1000) with the frontal cortex from FTLD-TDP type B patients. IHC experiment was done with Ventana automatic staining system and Optiview DAB detection kit with heat-induced epitope retrieval (boiling for 32 min in Tris-EDTA based solution CC1 buffer, Ventana). Fig from the lab of Dr. Neumann.

    Ready-to-use IHC kits available with TDP-43 antibody included
  • IHC staining of human brain using 80007-1-RR

    IHC staining of human brain using 80007-1-RR

    It's human subiculum (high-mag) and temporal cortex (medium-mag) from subject with limbic-predominant age-related TDP-43 encephalopathy neuropathologic change (LATE-NC). Staining provided by Pete Nelson and Ela Patel, U. Kentucky AD Research Center Neuropathology Core.

    Ready-to-use IHC kits available with TDP-43 antibody included
  • WB analysis of HeLa using 80007-1-RR

    WB analysis of HeLa using 80007-1-RR

    HeLa cells were subjected to SDS PAGE followed by western blot with 80007-1-RR (Phospho-TDP43 (Ser409/410) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of Jurkat using 80007-1-RR

    FC experiment of Jurkat using 80007-1-RR

    1X10^6 Jurkat cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.5 ug Anti-Human Phospho-TDP43 (Ser409/410) (80007-1-RR, Clone:6M10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 , or 0.5 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected infrontal cortex of FTLD-TDP type B case, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inCalyculin A treated Jurkat cells
Planning an IHC experiment? We recommend our IHCeasy TDP-43 Ready-To-Use IHC Kit. TDP-43 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80007-1-RR targets Phospho-TDP43 (Ser409/410) as part of a matched antibody pair:

MP00589-1: 80007-1-RR capture and 81350-1-RR detection (validated in Cytometric bead array)

MP00589-2: 80007-1-RR capture and 80002-1-RR detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, FC, IHC, ELISA Application Description
Cited ApplicationsWB, IF, IHC
Tested Reactivity Human, mouse
Cited Reactivityhuman, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name TAR DNA binding protein
Synonyms ALS10, Phospho-TDP43 (Ser409/410), TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43, tdp-43
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberNM_007375
Gene Symbol TARDBP
Gene ID (NCBI) 23435
RRIDAB_2882937
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13148
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176). 80007-1-RR is a recombinant rabbit monoclonal antibody recognizing TDP-43 only when phosphorylated at 409/410. Immunohistochemical analyses using this antibody only stain the insoluble inclusions in pathologic tissues without normal diffuse nuclear staining.

实验方案

Product Specific Protocols
WB protocol for Phospho-TDP43 (Ser409/410) antibody 80007-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Biochim Biophys Acta Mol Basis Dis

Phosphorylated TAR DNA-Binding Protein-43: Aggregation and Antibody-Based Inhibition.

Authors - Josephine C Esposto
View Article
humanWB,IF

bioRxiv

TDP-43 pathology links innate and adaptive immunity in amyotrophic lateral sclerosis

Authors - Baggio A Evangelista
View Article
mouseIF

Res Sq

Endogenous TDP-43 mislocalization in a novel knock-in mouse model reveals DNA repair impairment, inflammation, and neuronal senescence

Authors - Joy Mitra
View Article
mouseWB

Mol Neurobiol

A Panel of miRNA Biomarkers Common to Serum and Brain-Derived Extracellular Vesicles Identified in Mouse Model of Amyotrophic Lateral Sclerosis

Authors - Natasha Vassileff
View Article
mouseIF

J Exp Med

A TBK1 variant causes autophagolysosomal and motoneuron pathology without neuroinflammation in mice

Authors - David Brenner
View Article
humanIF,IHC

Brain Pathol

Detection of pTDP-43 via routine muscle biopsy: A promising diagnostic biomarker for amyotrophic lateral sclerosis

Authors - Qi-Jie Zhang
View Article