验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, Calyculin A treated cells |
Positive IHC detected in | frontal cortex of FTLD-TDP type B case, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
80007-1-RR targets Phospho-TDP43 (Ser409/410) in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB, IHC, IF |
经测试反应性 | human, mouse |
文献引用反应性 | human, mouse, zebrafish |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | TAR DNA binding protein |
别名 | TARDBP, TDP 43, TDP43, TDP-43, TAR DNA-binding protein 43 |
计算分子量 | 43 kDa |
观测分子量 | 45-50 kDa |
GenBank蛋白编号 | NM_007375 |
基因名称 | TDP-43 |
Gene ID (NCBI) | 23435 |
RRID | AB_2882937 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q13148 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176). 80007-1-RR is a recombinant rabbit monoclonal antibody recognizing TDP-43 only when phosphorylated at 409/410. Immunohistochemical analyses using this antibody only stain the insoluble inclusions in pathologic tissues without normal diffuse nuclear staining.
实验方案
Product Specific Protocols | |
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WB protocol for Phospho-TDP43 (Ser409/410) antibody 80007-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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J Biol Chem Regulation of TAR DNA binding protein 43 (TDP-43) homeostasis by cytosolic DNA accumulation | ||
Biochim Biophys Acta Mol Basis Dis Phosphorylated TAR DNA-Binding Protein-43: Aggregation and Antibody-Based Inhibition. | ||
bioRxiv TDP-43 pathology links innate and adaptive immunity in amyotrophic lateral sclerosis | ||
Res Sq Endogenous TDP-43 mislocalization in a novel knock-in mouse model reveals DNA repair impairment, inflammation, and neuronal senescence | ||
Mol Neurobiol A Panel of miRNA Biomarkers Common to Serum and Brain-Derived Extracellular Vesicles Identified in Mouse Model of Amyotrophic Lateral Sclerosis | ||
J Exp Med A TBK1 variant causes autophagolysosomal and motoneuron pathology without neuroinflammation in mice |