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验证数据展示

  • IHC staining of frontal cortex of FTLD-TDP type B case using 80007-1-RR (same clone as 80007-1-PBS)

    IHC staining of frontal cortex of FTLD-TDP type B case using 80007-1-RR (same clone as 80007-1-PBS)

    IHC results of Phospho-TDP43 (Ser409/410) rabbit recombinant antibody (80007-1-RR, 1000) with the frontal cortex from FTLD-TDP type B patients. IHC experiment was done with Ventana automatic staining system and Optiview DAB detection kit with heat-induced epitope retrieval (boiling for 32 min in Tris-EDTA based solution CC1 buffer, Ventana). Fig from the lab of Dr. Neumann. This data was developed using the same antibody clone with 80007-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TDP-43 antibody included
  • IHC staining of human brain using 80007-1-RR (same clone as 80007-1-PBS)

    IHC staining of human brain using 80007-1-RR (same clone as 80007-1-PBS)

    It's human subiculum (high-mag) and temporal cortex (medium-mag) from subject with limbic-predominant age-related TDP-43 encephalopathy neuropathologic change (LATE-NC). Staining provided by Pete Nelson and Ela Patel, U. Kentucky AD Research Center Neuropathology Core. This data was developed using the same antibody clone with 80007-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TDP-43 antibody included
  • WB analysis using 80007-1-RR (same clone as 80007-1-PBS)

    WB analysis using 80007-1-RR (same clone as 80007-1-PBS)

    Non-treated and Calyculin A treated cells were subjected to SDS PAGE followed by western blot with 80007-1-RR (Phospho-TDP43 (Ser409/410) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80007-1-PBS in a different storage buffer formulation.

  • Cytometric bead array in cell lysate using MP00589-1

    Cytometric bead array in cell lysate using MP00589-1

    Cytometric bead array in cell lysate using MP00589-1, phospho(409/410)-TDP43 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 80007-1-PBS. Detection antibody: 81350-1-PBS. Cell lysate(30μg/well): Non-treated HeLa and Calyculin A treated HeLa cells were lysed in 8M urea buffer and diluted in assay buffer. Non-related target Tubulin-Alpha Recombinant Matched Antibody Pair (MP00551-1) was served as control.

  • Cytometric bead array in cell lysate using MP00589-2

    Cytometric bead array in cell lysate using MP00589-2

    Cytometric bead array in cell lysate using MP00589-2, phospho(409/410)-TDP43 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 80007-1-PBS. Detection antibody: 80002-1-PBS. Cell lysate(30μg/well): Non-treated HeLa and Calyculin A treated HeLa cells were lysed in 8M urea buffer and diluted in assay buffer. Non-related target Tubulin-Alpha Recombinant Matched Antibody Pair (MP00551-1) was served as control.

Phospho-TDP43 (Ser409/410) Recombinant antibody, PBS Only (Capture)

Phospho-TDP43 (Ser409/410) Uni-rAbTM Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 80007-1-PBS

产品说明书

CloneNo. 6M10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Cytometric bead array, Indirect ELISA

别名

TARDBP, TDP 43, TDP43, TDP-43, TAR DNA-binding protein 43

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy TDP-43 Ready-To-Use IHC Kit. TDP-43 primary antibody included.

产品信息

80007-1-PBS targets Phospho-TDP43 (Ser409/410) as part of a matched antibody pair:

MP00589-1: 80007-1-PBS capture and 81350-1-PBS detection (validated in Cytometric bead array)

MP00589-2: 80007-1-PBS capture and 80002-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 TAR DNA binding protein
别名 TARDBP, TDP 43, TDP43, TDP-43, TAR DNA-binding protein 43
计算分子量 43 kDa
观测分子量 45-50 kDa
GenBank蛋白编号NM_007375
基因名称 TDP-43
Gene ID (NCBI) 23435
RRIDAB_2882937
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13148
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176). 80007-1-RR is a recombinant rabbit monoclonal antibody recognizing TDP-43 only when phosphorylated at 409/410. Immunohistochemical analyses using this antibody only stain the insoluble inclusions in pathologic tissues without normal diffuse nuclear staining.