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TDP-43 (C-terminal) Recombinant antibody

TDP-43 (C-terminal) Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

5D17

Cat no : 81350-1-RR

Print datasheet

Synonyms

TARDBP, TDP 43, TDP43, TDP-43, TAR DNA-binding protein 43

验证数据展示

  • WB analysis using 81350-1-RR

    WB analysis using 81350-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81350-1-RR (TDP-43 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 81350-1-RR

    IF Staining of HeLa using 81350-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TDP-43 (C-terminal) antibody (81350-1-RR, Clone: 5D17 ) at dilution of 1:5000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IHC staining of mouse brain using 81350-1-RR

    IHC staining of mouse brain using 81350-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81350-1-RR (TDP-43 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TDP-43 antibody included
  • IHC staining of mouse brain using 81350-1-RR

    IHC staining of mouse brain using 81350-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81350-1-RR (TDP-43 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TDP-43 antibody included
  • FC experiment of HeLa using 81350-1-RR

    FC experiment of HeLa using 81350-1-RR

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TDP-43 (C-terminal) (81350-1-RR, Clone:5D17) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 81350-1-RR

    Affinity and Kinetic Characterization of 81350-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81350-1-RR against Human TDP-43 (C-terminal) were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, NIH/3T3 cells, HSC-T6 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TDP-43 Ready-To-Use IHC Kit. TDP-43 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:2500-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

81350-1-RR targets TDP-43 (C-terminal) in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen fusion protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name TAR DNA binding protein
Synonyms TARDBP, TDP 43, TDP43, TDP-43, TAR DNA-binding protein 43
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC001487
Gene Symbol TDP-43
Gene ID (NCBI) 23435
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13148
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene. In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS).

实验方案

Product Specific Protocols
WB protocol for TDP-43 (C-terminal) antibody 81350-1-RRDownload protocol
IHC protocol for TDP-43 (C-terminal) antibody 81350-1-RRDownload protocol
IF protocol for TDP-43 (C-terminal) antibody 81350-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB

bioRxiv

Large-scale RNA-seq mining reveals ciclopirox triggers TDP-43 cryptic exons

Authors - Irika R Sinha
View Article