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验证数据展示

  • WB analysis using 81468-5-RR

    WB analysis using 81468-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81468-5-RR (SCD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 81468-5-RR

    IF Staining of MCF-7 using 81468-5-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SCD antibody (81468-5-RR, Clone: 241487E12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HepG2 using 81468-5-RR

    FC experiment of HepG2 using 81468-5-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug SCD Recombinant antibody (81468-5-RR, Clone:241487E12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of MCF-7 using 81468-5-RR

    FC experiment of MCF-7 using 81468-5-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug SCD Recombinant antibody (81468-5-RR, Clone:241487E12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 81468-5-RR

    Affinity and Kinetic Characterization of 81468-5-RR

    Biolayer interferometry (BLl) kinetic assays of 81468-5-RR against Human SCD were performed. The affinity constant is 1.83 nM.

SCD1 Recombinant antibody

SCD1 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 81468-5-RR

产品说明书

CloneNo. 241487E12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

SCD, Delta-9 desaturase, Delta(9)-desaturase, Delta(9) desaturase, Acyl-CoA desaturase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inHepG2 cells, MCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy SCD Ready-To-Use IHC Kit. SCD primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81468-5-RR targets SCD1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 SCD1 fusion protein Ag29156 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 stearoyl-CoA desaturase (delta-9-desaturase)
别名 SCD, Delta-9 desaturase, Delta(9)-desaturase, Delta(9) desaturase, Acyl-CoA desaturase
计算分子量 355 aa, 41 kDa
观测分子量28-42 kDa
GenBank蛋白编号BC005807
基因名称 SCD
Gene ID (NCBI) 6319
RRIDAB_3670496
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO00767
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SCD1 (stearoyl-CoA desaturase) is a microsomal fatty acid monodesaturase, which catalyses the committed step in the biosynthesis of mono-unsaturated fatty acids from saturated fatty acids (PMID:10946019). SCD1 and SCD2 are the main isoforms expressed in mouse liver and brain respectively (PMID:15907797). The formation of homodimers and oligomers is an intrinsic property of SCD proteins. SCD1 is a multi-pass membrane protein and detected double bands of 37-42 kDa. The degradation product of 28 kDa may be caused by a major cleavage site at the C-terminus (PMID:15610069, PMID: 9843580).

实验方案

Product Specific Protocols
WB protocol for SCD1 antibody 81468-5-RRDownload protocol
IF protocol for SCD1 antibody 81468-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols