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  • Featured Product
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ATG7 Recombinant antibody

ATG7 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

2M8

Cat no : 81760-1-RR

Print datasheet

Synonyms

APG7 LIKE, APG7L, ATG7, Autophagy related protein 7, DKFZp434N0735, GSA7, hAGP7

验证数据展示

  • WB analysis of HeLa using 81760-1-RR

    WB analysis of HeLa using 81760-1-RR

    HeLa cells were subjected to SDS PAGE followed by western blot with 81760-1-RR (ATG7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 81760-1-RR

    WB analysis of HepG2 using 81760-1-RR

    WB result of ATG7 antibody (81760-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATG7 transfected HepG2 cells.

  • IP experiment of HeLa using 81760-1-RR

    IP experiment of HeLa using 81760-1-RR

    IP result of anti-ATG7 (IP:81760-1-RR, 4ug; Detection:81760-1-RR 1:2000) with HeLa cells lysate 1720 ug.

  • IF Staining of HepG2 using 81760-1-RR

    IF Staining of HepG2 using 81760-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ATG7 antibody (81760-1-RR, Clone: 2M8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Affinity and Kinetic Characterization of 81760-1-RR

    Affinity and Kinetic Characterization of 81760-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81760-1-RR against Human ATG7 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, U2OS cells, Jurkat cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81760-1-RR targets ATG7 in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Tested Reactivity Human
Immunogen ATG7 fusion protein Ag0142 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ATG7 autophagy related 7 homolog (S. cerevisiae)
Synonyms APG7 LIKE, APG7L, ATG7, Autophagy related protein 7, DKFZp434N0735, GSA7, hAGP7
Calculated Molecular Weight 78 kDa
Observed Molecular Weight68-78 kDa
GenBank Accession NumberBC000091
Gene Symbol ATG7
Gene ID (NCBI) 10533
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95352
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Atg7 is an E1-like enzyme that is specifically involved in autophagosome formation and is essential for autophagy. As an autophagic-related protein it is required for linking to Atg12, Atg5 and Atg8, which are essential for Atg conjugation and autophagosome formation. Atg7 has been reported as an important regulator of autophagy with starvation-induced or chemotherapeutic agent treatment. The high expression level of ATG7 is related to the survival of patients with breast cancer. There are several isoforms of ATG7 protein ranged from 68 kDa to 78 kDa.

实验方案

Product Specific Protocols
WB protocol for ATG7 antibody 81760-1-RRDownload protocol
IF protocol for ATG7 antibody 81760-1-RRDownload protocol
IP protocol for ATG7 antibody 81760-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols