• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of HeLa using 81760-1-RR (same clone as 81760-1-PBS)

    WB analysis of HeLa using 81760-1-RR (same clone as 81760-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 81760-1-RR (ATG7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81760-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 81760-1-RR (same clone as 81760-1-PBS)

    WB analysis of HepG2 using 81760-1-RR (same clone as 81760-1-PBS)

    WB result of ATG7 antibody (81760-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATG7 transfected HepG2 cells. This data was developed using the same antibody clone with 81760-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 81760-1-RR (same clone as 81760-1-PBS)

    IP experiment of HeLa using 81760-1-RR (same clone as 81760-1-PBS)

    IP result of anti-ATG7 (IP:81760-1-RR, 4ug; Detection:81760-1-RR 1:2000) with HeLa cells lysate 1720 ug. This data was developed using the same antibody clone with 81760-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 81760-1-RR (same clone as 81760-1-PBS)

    IF Staining of HepG2 using 81760-1-RR (same clone as 81760-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ATG7 antibody (81760-1-RR, Clone: 2M8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 81760-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81760-1-PBS

    Affinity and Kinetic Characterization of 81760-1-PBS

    Biolayer interferometry (BLl) kinetic assays of 81760-1-RR against Human ATG7 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

ATG7 Recombinant antibody, PBS Only

ATG7 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 81760-1-PBS

产品说明书

CloneNo. 2M8

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IF/ICC, IP, Indirect ELISA

别名

APG7 LIKE, APG7L, ATG7, Autophagy related protein 7, DKFZp434N0735, GSA7, hAGP7

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

81760-1-PBS targets ATG7 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 Human
免疫原 ATG7 fusion protein Ag0142 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ATG7 autophagy related 7 homolog (S. cerevisiae)
别名 APG7 LIKE, APG7L, ATG7, Autophagy related protein 7, DKFZp434N0735, GSA7, hAGP7
计算分子量 78 kDa
观测分子量68-78 kDa
GenBank蛋白编号BC000091
基因名称 ATG7
Gene ID (NCBI) 10533
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO95352
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Atg7 is an E1-like enzyme that is specifically involved in autophagosome formation and is essential for autophagy. As an autophagic-related protein it is required for linking to Atg12, Atg5 and Atg8, which are essential for Atg conjugation and autophagosome formation. Atg7 has been reported as an important regulator of autophagy with starvation-induced or chemotherapeutic agent treatment. The high expression level of ATG7 is related to the survival of patients with breast cancer. There are several isoforms of ATG7 protein ranged from 68 kDa to 78 kDa.