- Featured Product
- KD/KO Validated
ATG7 Recombinant antibody
ATG7 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human and More (1)
Applications
WB, IF/ICC, IP, ELISA
Conjugate
Unconjugated
CloneNo.
2M8
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HepG2 cells, U2OS cells, Jurkat cells |
Positive IP detected in | HeLa cells |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
81760-1-RR targets ATG7 in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human samples.
Tested Applications | WB, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | Human |
Cited Reactivity | human |
Immunogen | ATG7 fusion protein Ag0142 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | ATG7 autophagy related 7 homolog (S. cerevisiae) |
Synonyms | APG7 LIKE, APG7L, ATG7, Autophagy related protein 7, DKFZp434N0735, GSA7, hAGP7 |
Calculated Molecular Weight | 78 kDa |
Observed Molecular Weight | 68-78 kDa |
GenBank Accession Number | BC000091 |
Gene Symbol | ATG7 |
Gene ID (NCBI) | 10533 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | O95352 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for ATG7 antibody 81760-1-RR | Download protocol |
IF protocol for ATG7 antibody 81760-1-RR | Download protocol |
IP protocol for ATG7 antibody 81760-1-RR | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |