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验证数据展示

  • WB analysis of HEK-293 using 81768-1-RR

    WB analysis of HEK-293 using 81768-1-RR

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 81768-1-RR (EIF2AK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 81768-1-RR

    WB analysis of HEK-293 using 81768-1-RR

    WB result of EIF2AK1 antibody (81768-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF2AK1 transfected HEK-293 cells.

  • Affinity and Kinetic Characterization of 81768-1-RR

    Affinity and Kinetic Characterization of 81768-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81768-1-RR against Human EIF2AK1 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

EIF2AK1 Recombinant monoclonal antibody

EIF2AK1 Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 81768-1-RR

产品说明书

CloneNo. 3J9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

3J9, EC:2.7.11.1, Eukaryotic translation initiation factor 2-alpha kinase 1, HCR, Heme controlled repressor

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, MCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy EIF2AK1 Ready-To-Use IHC Kit. EIF2AK1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81768-1-RR targets EIF2AK1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag14236

Product name: Recombinant human EIF2AK1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 281-630 aa of BC006524

Sequence: FAEPTPEKEKRFGESDTENQNNKSVKYTTNLVIRESGELESTLELQENGLAGLSASSIVEQQLPLRRNSHLEESFTSTEESSEENVNFLGQTEAQYHLMLHIQMQLCELSLWDWIVERNKRGREYVDESACPYVMANVATKIFQELVEGVFYIHNMGIVHRDLKPRNIFLHGPDQQVKIGDFGLACTDILQKNTDWTNRNGKRTPTHTSRVGTCLYASPEQLEGSEYDAKSDMYSLGVVLLELFQPFGTEMERAEVLTGLRTGQLPESLRKRCPVQAKYIQHLTRRNSSQRPSAIQLLQSELFQNSGNVNLTLQMKIIEQEKEIAELKKQLNLLSQDKGVRDDGKDGGVG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 eukaryotic translation initiation factor 2-alpha kinase 1
别名 3J9, EC:2.7.11.1, Eukaryotic translation initiation factor 2-alpha kinase 1, HCR, Heme controlled repressor
计算分子量 630 aa, 71 kDa
观测分子量 71 kDa
GenBank蛋白编号BC006524
基因名称 EIF2AK1
Gene ID (NCBI) 27102
RRIDAB_2935578
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9BQI3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Eukaryotic initiation factor 2a (eIF2a), which is a critical component in the formation of the translation initiation ternary complex (eIF2a/GTP/methionyl transfer RNA), plays a key role in the ER stress response. EIF2AK1, which is activated by heme deficiency and other stimuli, is a major kinase that phosphorylates EIF2-alpha. Phosphorylation of the alpha subunit of EIF2 downregulates protein synthesis in response to various stress conditions.

实验方案

Product Specific Protocols
WB protocol for EIF2AK1 antibody 81768-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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