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  • Featured Product
  • KD/KO Validated

EIF2AK1 Recombinant antibody, PBS Only

EIF2AK1 Uni-rAbTM Recombinant Antibody for Indirect ELISA, WB

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Indirect ELISA, WB

Conjugate

Unconjugated

CloneNo.

3J9

Cat no : 81768-1-PBS

Print datasheet

Synonyms

Heme regulated inhibitor, Heme controlled repressor, HCR, Eukaryotic translation initiation factor 2-alpha kinase 1, EC:2.7.11.1

验证数据展示

  • WB analysis of HEK-293 using 81768-1-RR (same clone as 81768-1-PBS)

    WB analysis of HEK-293 using 81768-1-RR (same clone as 81768-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 81768-1-RR (EIF2AK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81768-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 81768-1-RR (same clone as 81768-1-PBS)

    WB analysis of HEK-293 using 81768-1-RR (same clone as 81768-1-PBS)

    WB result of EIF2AK1 antibody (81768-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF2AK1 transfected HEK-293 cells. This data was developed using the same antibody clone with 81768-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81768-1-RR

    Affinity and Kinetic Characterization of 81768-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81768-1-RR against Human EIF2AK1 were performed. The affinity constant is below 1 pM.

产品信息

81768-1-PBS targets EIF2AK1 in Indirect ELISA, WB applications and shows reactivity with human samples.

Tested Applications Indirect ELISA, WB Application Description
Tested Reactivity human
Immunogen EIF2AK1 fusion protein Ag14236 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name eukaryotic translation initiation factor 2-alpha kinase 1
Synonyms Heme regulated inhibitor, Heme controlled repressor, HCR, Eukaryotic translation initiation factor 2-alpha kinase 1, EC:2.7.11.1
Calculated Molecular Weight 630 aa, 71 kDa
Observed Molecular Weight 71 kDa
GenBank Accession NumberBC006524
Gene Symbol EIF2AK1
Gene ID (NCBI) 27102
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BQI3
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Eukaryotic initiation factor 2a (eIF2a), which is a critical component in the formation of the translation initiation ternary complex (eIF2a/GTP/methionyl transfer RNA), plays a key role in the ER stress response. EIF2AK1, which is activated by heme deficiency and other stimuli, is a major kinase that phosphorylates EIF2-alpha. Phosphorylation of the alpha subunit of EIF2 downregulates protein synthesis in response to various stress conditions. EIF2AK1 has some isoforms with 630aa, 577aa and 422aa.