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验证数据展示

  • WB analysis using 81903-1-RR

    WB analysis using 81903-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81903-1-RR (DDX3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 81903-1-RR

    WB analysis of HepG2 using 81903-1-RR

    WB result of DDX3 antibody (81903-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDX3 transfected HepG2 cells.

  • IF Staining of HepG2 using 81903-1-RR

    IF Staining of HepG2 using 81903-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DDX3 antibody (81903-1-RR, Clone: 5C12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HepG2 using 81903-1-RR

    FC experiment of HepG2 using 81903-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug DDX3 Recombinant antibody (81903-1-RR, Clone:5C12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

DDX3 Recombinant antibody

DDX3 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 81903-1-RR

产品说明书

CloneNo. 5C12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, FC (Intra), ELISA

别名

DDX3X, DDX14, DBX, CAP-Rf, ATP-dependent RNA helicase DDX3X

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, K-562 cells, mouse brain tissue, rat brain tissue
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy DDX3 Ready-To-Use IHC Kit. DDX3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81903-1-RR targets DDX3 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 DDX3 fusion protein Ag1614 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked
别名 DDX3X, DDX14, DBX, CAP-Rf, ATP-dependent RNA helicase DDX3X
计算分子量 73 kDa
观测分子量75 kDa
GenBank蛋白编号BC011819
基因名称 DDX3
Gene ID (NCBI) 1654
RRIDAB_2935591
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO00571
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DDX3X, also named as DBX, DDX3 and HLP2, belongs to the DEAD box helicase family and DDX3/DED1 subfamily. It is ATP-dependent RNA helicase. DDX3X acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. It is also involved in HIV-1 replication. DDX3X interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location. 73 kDa is the target band. 60-65 kDa is some other isoform. This antibody can bind both DDX3X and DDX3Y for the close sequences.

实验方案

Product Specific Protocols
WB protocol for DDX3 antibody 81903-1-RRDownload protocol
IF protocol for DDX3 antibody 81903-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols