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DDX3 Recombinant antibody, PBS Only

DDX3 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5C12

Cat no : 81903-1-PBS

Print datasheet

Synonyms

DDX3X, DDX14, DBX, CAP-Rf, ATP-dependent RNA helicase DDX3X

验证数据展示

  • WB analysis using 81903-1-RR (same clone as 81903-1-PBS)

    WB analysis using 81903-1-RR (same clone as 81903-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81903-1-RR (DDX3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81903-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 81903-1-RR (same clone as 81903-1-PBS)

    WB analysis of HepG2 using 81903-1-RR (same clone as 81903-1-PBS)

    WB result of DDX3 antibody (81903-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDX3 transfected HepG2 cells. This data was developed using the same antibody clone with 81903-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 81903-1-RR (same clone as 81903-1-PBS)

    IF Staining of HepG2 using 81903-1-RR (same clone as 81903-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DDX3 antibody (81903-1-RR, Clone: 5C12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81903-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 81903-1-RR (same clone as 81903-1-PBS)

    FC experiment of HepG2 using 81903-1-RR (same clone as 81903-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug DDX3 Recombinant antibody (81903-1-RR, Clone:5C12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81903-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy DDX3 Ready-To-Use IHC Kit. DDX3 primary antibody included.

产品信息

81903-1-PBS targets DDX3 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen DDX3 fusion protein Ag1614 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked
Synonyms DDX3X, DDX14, DBX, CAP-Rf, ATP-dependent RNA helicase DDX3X
Calculated Molecular Weight 73 kDa
Observed Molecular Weight75 kDa
GenBank Accession NumberBC011819
Gene Symbol DDX3
Gene ID (NCBI) 1654
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00571
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DDX3X, also named as DBX, DDX3 and HLP2, belongs to the DEAD box helicase family and DDX3/DED1 subfamily. It is ATP-dependent RNA helicase. DDX3X acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. It is also involved in HIV-1 replication. DDX3X interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location. 73 kDa is the target band. 60-65 kDa is some other isoform. This antibody can bind both DDX3X and DDX3Y for the close sequences.