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DDX3 Recombinant antibody, PBS Only

DDX3 Recombinant Antibody for WB,Indirect ELISA,IF

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB,Indirect ELISA,IF

Conjugate

Unconjugated

CloneNo.

5C12

Cat No : 81903-1-PBS

Print datasheet

Synonyms

DBX, DDX14, DDX3, DDX3X, DEAD box, X isoform, Helicase like protein 2, HLP2

验证数据展示

  • WB analysis using 81903-1-RR (same clone as 81903-1-PBS)

    WB analysis using 81903-1-RR (same clone as 81903-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81903-1-RR (DDX3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81903-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 81903-1-RR (same clone as 81903-1-PBS)

    IF Staining of HepG2 using 81903-1-RR (same clone as 81903-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DDX3 antibody (81903-1-RR, Clone: 5C12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81903-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy DDX3 Ready-To-Use IHC Kit. DDX3 primary antibody included.

产品信息

81903-1-PBS targets DDX3 in WB, IF, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB,Indirect ELISA,IF Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen DDX3 fusion protein Ag1614 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked
Synonyms DBX, DDX14, DDX3, DDX3X, DEAD box, X isoform, Helicase like protein 2, HLP2
Calculated Molecular Weight 73 kDa
Observed Molecular Weight75 kDa
GenBank Accession NumberBC011819
Gene Symbol DDX3X
Gene ID (NCBI) 1654
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00571
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

DDX3X, also named as DBX, DDX3 and HLP2, belongs to the DEAD box helicase family and DDX3/DED1 subfamily. It is ATP-dependent RNA helicase. DDX3X acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. It is also involved in HIV-1 replication. DDX3X interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location. 73 kDa is the target band. 60-65 kDa is some other isoform. This antibody can bind both DDX3X and DDX3Y for the close sequences.